基于分子生物学的药用甘草品种界定研究
[Study on circumscription of medicinal licorice species based on molecular biology].
作者信息
Liu Chun-sheng, Wang Peng-yi, Wang Wen-quan
机构信息
School of Pharmacology, Beijing University of Chinese Medicine, Beijing 100029, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2005 Nov;30(22):1736-8.
PMID:16468366
Abstract
OBJECTIVE
Elucidate the medicinal licorice species circumscription in order to develop licorice resources in China and select new cultivars.
METHOD
PCR amplification, DNA sequencing and cladistic analysis.
RESULT
Acquire the ITS sequences and phylogenetic tree of 8 licorice species.
CONCLUSION
According to results Glycyrrhiza eurycarpa and G. glabra var. glandulosa should be combined into G. and G. glabra respectively and G. egladulosa is an independent species.
摘要
目的
阐明药用甘草的物种界定，以便开发中国的甘草资源并选育新品种。
方法
聚合酶链反应（PCR）扩增、DNA测序和分支分析。
结果
获得8种甘草的内转录间隔区（ITS）序列和系统发育树。
结论
根据结果，光果甘草变种腺毛甘草应分别合并到光果甘草和胀果甘草中，而圆果甘草是一个独立的物种。
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