使用针对白色念珠菌分离株中分泌天冬氨酸蛋白酶（SAP）的特异性引物通过聚合酶链反应分析来检测分泌天冬氨酸蛋白酶的分布情况。

Distribution of secreted aspartyl proteinases using a polymerase chain reaction assay with SAP specific primers in Candida albicans isolates.

作者信息

Kalkanci A, Bozdayi G, Biri A, Kustimur S

机构信息

Department of Microbiology, Faculty of Medicine, Gazi University, 06510 Ankara, Turkey.

出版信息

Folia Microbiol (Praha). 2005;50(5):409-13. doi: 10.1007/BF02931422.

DOI:10.1007/BF02931422

PMID:16475500

Abstract

Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans strains isolated from blood cultures. SAP1-SAP3 were detected in 37 of 40 (92.5%), SAP4 were detected in 3/40 (7.5%), SAP5 in 3/40 (7.5%), SAP6 in 5/40 (12.5%) of C. albicans strains isolated from vaginal swab cultures. Sap1, Sap2 and Sap3 isoenzymes were found to be related to the vaginopathic potential of C. albicans; Sap4, Sap5 and Sap6 isoenzymes were found to be correlated with systemic infections.

摘要

采用聚合酶链反应（PCR）分析中针对分泌天冬氨酸蛋白酶（Sap）的特异性引物，确定了不同白色念珠菌分离株中分泌天冬氨酸蛋白酶（Sap）的分布情况。在从血培养物中分离出的40株白色念珠菌菌株中，13株（32.5%）检测到SAP1、SAP2和SAP3，38/40（95%）检测到SAP4，30/40（75%）检测到SAP5，23/40（57.5%）检测到SAP6。在从阴道拭子培养物中分离出的40株白色念珠菌菌株中，37株（92.5%）检测到SAP1 - SAP3，3/40（7.5%）检测到SAP4，3/40（7.5%）检测到SAP5，5/40（12.5%）检测到SAP6。发现Sap1、Sap2和Sap3同工酶与白色念珠菌的阴道致病潜力有关；发现Sap4、Sap5和Sap6同工酶与全身感染相关。