用于鉴定金黄色葡萄球菌的试管凝固酶试验解读
Interpretation of the tube coagulase test for identification of Staphylococcus aureus.
作者信息
Sperber W H, Tatini S R
出版信息
Appl Microbiol. 1975 Apr;29(4):502-5. doi: 10.1128/am.29.4.502-505.1975.
Abstract
The tube coagulase test is a valid means of identifying Staphylococcus auerus, provided that only a firm clot that does not move when the tube is tipped is considered a positive reaction. The widely promulgated interpretation that all degrees of clotting in coagulase plasma are a positive identification of S. auerus was disproved by the use of other tests such as anaerobic glucose fermentation, thermonuclease production, and lysostaphin sensitivity. It was found that the source of supply of the coagulase plasma is a factor in the occurrence of false-positive coagulase test results. The use of a mixture of pig and rabbit plasma in the tube coagulase test is also discussed.
摘要
试管凝固酶试验是鉴定金黄色葡萄球菌的有效方法，前提是只有当试管倾斜时不移动的坚实凝块才被视为阳性反应。通过使用其他试验，如厌氧葡萄糖发酵、耐热核酸酶产生和溶葡萄球菌素敏感性试验，证明了广泛传播的关于凝固酶血浆中所有程度的凝血都是金黄色葡萄球菌阳性鉴定的解释是错误的。发现凝固酶血浆的供应来源是导致凝固酶试验结果出现假阳性的一个因素。还讨论了在试管凝固酶试验中使用猪和兔血浆混合物的情况。
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