Terenina N B, Tolstenkov O, Fagerholm H-P, Serbina E A, Vodjanitskaja S N, Gustafsson M K S
Institute of Parasitology, Russian Academy of Sciences, Lenin Avenue 33, 117071 Moscow, Russia.
Tissue Cell. 2006 Apr;38(2):151-7. doi: 10.1016/j.tice.2006.01.003. Epub 2006 Feb 21.
The spatial relationship between the musculature and the NADPH-diaphorase (NADPH-d) activity, 5-HT and FMRFamide immunoreactivities in redia, cercaria and adult Echinoparyphium aconiatum was studied using scanning electron microscopy (SEM), NADPH-d histochemistry, immunocytochemistry, and confocal scanning laser microscopy (CSLM). TRITC-conjugated phalloidin was used to stain the musculature. Staining for NADPH-d was observed in the central (CNS) and peripheral nervous system (PNS) of all three stages. NADPH-d positive nerves occurred very close to muscle fibres. 5-HT-immunoreactive (5-HT-IR) nerve cells and fibres occurred in the CNS and PNS and close to muscle fibres. FMRFamide-IR nerve fibres were observed in the CNS and PNS of adult worms. This is the first time, the presence of the NADPH-d has been demonstrated in the larval as well as the adult stages of a fluke.
利用扫描电子显微镜（SEM）、NADPH-d组织化学、免疫细胞化学和共聚焦扫描激光显微镜（CSLM），研究了棘口吸虫雷蚴、尾蚴和成虫阶段肌肉组织与NADPH-黄递酶（NADPH-d）活性、5-羟色胺（5-HT）和FMRF酰胺免疫反应性之间的空间关系。用TRITC标记的鬼笔环肽对肌肉组织进行染色。在所有三个阶段的中枢神经系统（CNS）和外周神经系统（PNS）中均观察到NADPH-d染色。NADPH-d阳性神经非常靠近肌纤维。5-HT免疫反应性（5-HT-IR）神经细胞和纤维出现在中枢神经系统和外周神经系统中，并靠近肌纤维。在成虫的中枢神经系统和外周神经系统中观察到FMRF酰胺免疫反应性神经纤维。这是首次在吸虫的幼虫和成虫阶段均证实存在NADPH-d。
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