联合分支DNA与传统乙肝病毒聚合酶链反应检测法用于检测乙肝e抗原阳性慢性乙型肝炎患者血清中的乙肝病毒DNA

Combined branched-DNA and conventional HBV PCR assays for detection of serum HBV-DNA in hepatitis B e antigen-positive chronic hepatitis B patients.

作者信息

Ozdarendeli Aykut, Toroman Zulal Asci, Bulut Yasemin, Demirbag Kutbeddin, Kalkan Ahmet, Ozden Mehmet, Kilic Suleyman Sirri

机构信息

Department of Microbiology and Clinic Microbiology, College of Medicine, University of Firat, Elazig, Turkey.

出版信息

Hepatogastroenterology. 2006 Jan-Feb;53(67):106-9.

PMID:16506386

Abstract

BACKGROUND/AIMS: Monitoring of HBV replication level is very useful for the management of patients with chronic HBV. However, the use of the correct tools to quantify HBV-DNA levels in serum and monitor the replication of HBV is of paramount importance in terms of diagnosis, and antiviral treatment of patients with chronic HBV infection. The aim of this study was to combine the bDNA assay and HBV PCR to improve detection of viremia the patients with HBeAg-positive chronic hepatitis B infection.

METHODOLOGY

In this study, 67 HBeAg-positive chronic hepatitis B patients were analyzed to determine viremia level using bDNA and HBV PCR assays.

RESULTS

Sixty-four patients with HBeAg-positive chronic hepatitis B showed positivity by conventional HBV PCR, whereas 56 subjects with HBeAg-positive chronic hepatitis B showed HBV-DNA levels by bDNA.

CONCLUSIONS

The results indicated that it is reasonable to use the bDNA assay to determine HBV replicative activity first, and use conventional HBV-PCR for HBeAg-positive chronic hepatitis B patient samples that are negative in bDNA assay.

摘要

背景/目的：监测HBV复制水平对慢性HBV患者的管理非常有用。然而，使用正确的工具来定量血清中的HBV-DNA水平并监测HBV复制，对于慢性HBV感染患者的诊断和抗病毒治疗至关重要。本研究的目的是将分支DNA检测法（bDNA检测法）与HBV聚合酶链反应（PCR）相结合，以提高对HBeAg阳性慢性乙型肝炎感染患者病毒血症的检测。