泥螈视网膜中丝氨酸对神经元抑制作用的特征分析。

Characterization of serine's inhibitory action on neurons in the mudpuppy retina.

作者信息

Slaughter M M, Miller R F

机构信息

Department of Biophysical Sciences, School of Medicine, State University of New York, Buffalo 14214.

出版信息

Neuroscience. 1991;41(2-3):817-25. doi: 10.1016/0306-4522(91)90372-u.

DOI:10.1016/0306-4522(91)90372-u

PMID:1651465

Abstract

Experiments were performed in the superfused retina-eyecup of mudpuppies using intracellular electrophysiological techniques to evaluate the effects of serine on amacrine and ganglion cells. Serine was found to have a dose-dependent inhibitory effect mediated by the opening of chloride channels. Serine appears to act on a glycine receptor based on the observations that: (1) serine's effect is blocked by strychnine but not by bicuculline or picrotoxin, (2) in the presence of saturating glycine concentrations serine had no effect on membrane voltage or conductance, and (3) cells inhibited by serine were always sensitive to glycine, but not always sensitive to GABA. High pressure liquid chromatography measurements disclose that there is a high concentration of extracellular serine in the retina. The data indicate that serine could act as an inhibitory neurotransmitter.

摘要

使用细胞内电生理技术，在泥螈的灌注视网膜-眼杯中进行实验，以评估丝氨酸对无长突细胞和神经节细胞的影响。发现丝氨酸具有由氯离子通道开放介导的剂量依赖性抑制作用。基于以下观察结果，丝氨酸似乎作用于甘氨酸受体：（1）丝氨酸的作用被士的宁阻断，但不被荷包牡丹碱或苦味毒阻断；（2）在甘氨酸浓度饱和的情况下，丝氨酸对膜电压或电导没有影响；（3）被丝氨酸抑制的细胞总是对甘氨酸敏感，但并非总是对GABA敏感。高压液相色谱测量显示，视网膜中细胞外丝氨酸浓度很高。数据表明丝氨酸可能作为一种抑制性神经递质。