Johnston Stephanie P, Pieniazek Norman J, Xayavong Maniphet V, Slemenda Susan B, Wilkins Patricia P, da Silva Alexandre J
Division of Parasitic Diseases, M.S. F-36, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, 4770 Buford Highway NE, Atlanta, Georgia 30341-3724, USA.
J Clin Microbiol. 2006 Mar;44(3):1087-9. doi: 10.1128/JCM.44.3.1087-1089.2006.
We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal.
我们比较了巢式聚合酶链反应(PCR)检测法与吉姆萨染色血涂片显微镜检查法,以检测和鉴定血液标本中的疟原虫种类。当显微镜检查结果不明确时,PCR比显微镜检查更敏感,并且能够在种属水平上鉴定疟原虫。