用于近距离位点多位点诱变的组合引物聚合酶链反应
Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites.
作者信息
Jensen Pia Hønnerup, Weilguny Dietmar
机构信息
Natlmmune A/S, Copenhagen, Denmark.
出版信息
J Biomol Tech. 2005 Dec;16(4):336-40.
Abstract
We describe a rapid and efficient polymerase chain reaction procedure for multi-site-directed mutagenesis for cases in which the sites to be mutated are in close proximity. The combination primer polymer chain reaction method is based on a multi-site directed mutagenesis protocol together with a splicing by overlapping extension polymerase chain reaction protocol. several different combinations of multiple mutations were successfully performed with this method and are reported in this study.
摘要
我们描述了一种快速有效的聚合酶链反应程序，用于多个待突变位点彼此靠近的多位点定向诱变情况。组合引物聚合酶链反应方法基于多位点定向诱变方案以及重叠延伸聚合酶链反应剪接方案。用该方法成功进行了几种不同的多位点突变组合，并在本研究中报告。
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