棉籽壳木聚糖中展青霉素抑制活性的研究
Demonstration of aflatoxin inhibitory activity in a cotton seed coat xylan.
出版信息
Appl Environ Microbiol. 1995 Dec;61(12):4409-12. doi: 10.1128/aem.61.12.4409-4412.1995.
An inhibitor of aflatoxin biosynthesis localized in the seed coats of developing cotton was partially purified and characterized. Aqueous extracts from 25-day postanthesis seed coat tissue inhibited aflatoxin (B(inf1)) production in liquid cultures of Aspergillus flavus AF13. Inhibition was concentration dependent, with a 50% effective dose of 173 (mu)g of crude extract per ml of medium. The inhibitor was neutral in charge. Two active fractions were obtained from crude preparations by gel filtration chromatography (BioGel P-100). The purest fraction eluted in the void volume. Carbohydrate composition analysis of this void volume inhibitor indicated a composition of xylose (>90%) and mannose. Aflatoxin production in vitro was inversely related to inhibitor concentration in the fermentation medium (log of aflatoxin versus log of [inhibitor]; r(sup2) = 0.82; P < 0.002). The void volume inhibitor had a 50% effective dose of 6.2 (mu)g/ml, a 28-fold purification of the inhibitor material. These data support the hypothesis that seed coat inhibitory activity is associated with a cottonseed-specific xylan.
一种定位于发育中棉花种皮的黄曲霉毒素生物合成抑制剂被部分纯化并进行了特性鉴定。来自授粉后 25 天的种皮组织的水提取物抑制了黄曲霉（AF13）在液体培养基中的产毒作用。抑制作用呈浓度依赖性，在培养基中 50%有效剂量为 173μg 粗提取物/ml。抑制剂带中性电荷。粗制剂经凝胶过滤层析（BioGel P-100）得到两个活性级分。最纯级分在空隙体积中洗脱。该空隙体积抑制剂的碳水化合物组成分析表明其组成主要为木糖（>90%）和甘露糖。体外产毒与发酵培养基中抑制剂浓度呈负相关（黄曲霉毒素对[抑制剂]的对数；r(sup2) = 0.82；P < 0.002）。空隙体积抑制剂的 50%有效剂量为 6.2μg/ml，抑制剂物质的纯化倍数为 28 倍。这些数据支持这样一种假设，即种皮抑制活性与棉籽特异性木聚糖有关。
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