[氯乙烯单体暴露工人DNA损伤与代谢酶多态性的关系研究]

[Study on the relationship between DNA damage and polymorphisms of metabolizing enzymes of vinyl chloride monomer-exposed workers].

作者信息

Zhu Shou-min, Cheng Wei, Du Feng-ren, Wang Ai-hong

机构信息

School of Public Health, Fudan University, Shanghai 200032, China.

出版信息

Wei Sheng Yan Jiu. 2005 Nov;34(6):655-7.

PMID:16535827

Abstract

OBJECTIVE

To explore the relationship between DNA damage induced by vinyl chloride monomer (VCM) and polymorphisms of metabolizing enzymes of VCM.

METHODS

Comet assay was employed to detect DNA damage, the workers were divided into two groups based on the status of DNA damage. Case-control design was used to investigate the relationship between the genetic polymorphisms of metabolizing enzymes and DNA damage. Genotypes of CYP2E1 c1/c2 and mEH4 His139Arg were identified by the PCR-RFLP, null genotypes of GST T1 and GST M1 were detected by PCR.

RESULTS

The genotypes of CYP2E1 c1c2 and c2c2 were significantly associated with DNA damages (P < 0.01). A prominent risk increasing of DNA damage was observed for those individuals having a high or low VCM exposure and possessing the CYP2E1 c1c2 and c2c2 genotypes (OR 4.92, 95% CI 1.35-13.85 and OR 2.57, 95% CI 1.01-6.59).

CONCLUSION

Cumulative exposure dose and polymorphism of metabolizing enzymes may modulate the DNA damage of VCM-exposed workers. possessing the CYP2E1 c1c2 and c2c2 genotypes (OR 4.92, 95% CI 1.35-13.85 and OR 2.57, 95% CI 1.01-6.59).

CONCLUSION

Cumulative exposure dose and polymorphism of metabolizing enzymes may modulate the DNA damage of VCM-exposed workers.

摘要

目的

探讨氯乙烯单体（VCM）诱导的DNA损伤与VCM代谢酶多态性之间的关系。

方法

采用彗星试验检测DNA损伤，根据DNA损伤状况将工人分为两组。采用病例对照设计研究代谢酶基因多态性与DNA损伤之间的关系。通过PCR-RFLP鉴定CYP2E1 c1/c2和mEH4 His139Arg的基因型，通过PCR检测GST T1和GST M1的缺失基因型。

结果

CYP2E1 c1c2和c2c2基因型与DNA损伤显著相关（P < 0.01）。对于VCM暴露水平高或低且具有CYP2E1 c1c2和c2c2基因型的个体，观察到DNA损伤的风险显著增加（比值比4.92，95%可信区间1.35 - 13.85；比值比2.57，95%可信区间1.01 - 6.59）。