人视网膜中精液蛋白的特征分析。
Characterization of semenogelin proteins in the human retina.
作者信息
Bonilha Vera L, Rayborn Mary E, Shadrach Karen, Lundwall Ake, Malm Johan, Bhattacharya Sanjoy K, Crabb John W, Hollyfield Joe G
机构信息
Department of Ophthalmic Research, The Cole Eye Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
出版信息
Exp Eye Res. 2006 Jul;83(1):120-7. doi: 10.1016/j.exer.2005.11.011. Epub 2006 Mar 20.
Semenogelin I and II are the major proteins present in semen coagulum. In the present study, semenogelin I and II were detected in human RPE lysates by proteomic analysis. We further analyzed the expression of these proteins in the retinal cells in vivo and in vitro. Western blots detected semenogelin I and II in both RPE and neural retina while the vitreous contained only SgII. Cryo and paraffin sections of human retina were processed for both immunofluorescence and DAB reaction with an antibody that recognizes both forms of semenogelin proteins. Retina and RPE total lysates were evaluated for the presence of these proteins and in a human RPE cell line (D407). Both proteins were detected by western blot in human RPE and in D407 cell lysates. Immunoreactivity was detected in the ganglion cell and photoreceptor layer of the retina. Our data support the expression of semenogelin I and II in the human retina in several different compartments. Further studies towards addressing the function of these proteins in the retina are in progress.
精液凝固蛋白I和II是精液凝块中的主要蛋白质。在本研究中，通过蛋白质组学分析在人视网膜色素上皮（RPE）裂解物中检测到了精液凝固蛋白I和II。我们进一步分析了这些蛋白质在体内和体外视网膜细胞中的表达情况。蛋白质免疫印迹法在RPE和神经视网膜中均检测到了精液凝固蛋白I和II，而玻璃体中仅含有精液凝固蛋白II。对人视网膜的冷冻切片和石蜡切片进行了免疫荧光处理以及与识别两种形式精液凝固蛋白的抗体进行DAB反应。对视网膜和RPE的总裂解物以及人RPE细胞系（D407）进行了这些蛋白质存在情况的评估。通过蛋白质免疫印迹法在人RPE和D407细胞裂解物中均检测到了这两种蛋白质。在视网膜的神经节细胞层和光感受器层检测到了免疫反应性。我们的数据支持精液凝固蛋白I和II在人视网膜的几个不同区域中的表达。针对这些蛋白质在视网膜中的功能的进一步研究正在进行中。
Zotero 插件