布拉酵母菌对梗阻性黄疸大鼠细菌移位的影响。
The effects of Saccharomyces boulardii on bacterial translocation in rats with obstructive jaundice.
作者信息
Geyik Mehmet Faruk, Aldemir Mustafa, Hosoglu Salih, Ayaz Celal, Satilmis Selda, Buyukbayram Huseyin, Kokoglu Omer Faruk
机构信息
Department of Clinical Microbiology and Infectious Diseases, Dicle University Medical Faculty, Diyarbakir, Turkey.
出版信息
Ann R Coll Surg Engl. 2006 Mar;88(2):176-80. doi: 10.1308/003588406X94986.
INTRODUCTION
The aim of this study was to investigate the effect of Saccharomyces boulardii treatment on preventing bacterial translocation in an obstructive jaundice animal model.
MATERIALS AND METHODS
Sixty adult rats were divided into five groups: group 1 - the sham-operated group; group 2 - the common bile duct ligation group; group 3 - the S. boulardii group; group 4 - the ampicillin-sulbaktam group; and group 5 - the S. boulardii plus ampicillin-sulbaktam group. The saline, antibiotics and S. boulardii were given, respectively, for a 7-day period as a single dose per day via temporary orogastric intubation. Seven days following the obstructive jaundice, the animal had laparatomy under sterile conditions. Segments of ileum were removed for histopathological examination. Blood, liver, spleen and mesenteric lymph nodes were taken for microbiological culture.
RESULTS
Bacterial translocation rates were 0% in the sham-operated group, 83% in group 2, 42% in group 3, 42% in group 4 and 33% in group 5. Bacterial translocation significantly increased in group 2 compared to groups 3, 4 and 5 (P = 0.001). The bacterial counts (CFU/g) of group 2 were significantly higher than those of groups 3, 4 and 5 (P = 0.001). Histopathological examination of ileum specimens revealed a significant decrease in the heights of villi in groups 2-5 compared to the sham-operated group (P = 0.001). The mean villus height in groups 3 and 5 was significantly higher than that of group 4 (P = 0.001).
CONCLUSIONS
S. boulardii was found to be effective in the successful control of translocation and improvement of intestinal barrier function.
引言
本研究旨在探讨布拉酵母菌治疗对阻塞性黄疸动物模型中预防细菌移位的作用。
材料与方法
60只成年大鼠分为五组：第1组——假手术组；第2组——胆总管结扎组；第3组——布拉酵母菌组；第4组——氨苄西林舒巴坦组；第5组——布拉酵母菌加氨苄西林舒巴坦组。通过临时经口胃管插管，分别给予生理盐水、抗生素和布拉酵母菌，为期7天，每天单剂量给药。阻塞性黄疸7天后，动物在无菌条件下进行剖腹手术。取出回肠段进行组织病理学检查。采集血液、肝脏、脾脏和肠系膜淋巴结进行微生物培养。
结果
假手术组细菌移位率为0%，第2组为83%，第3组为42%，第4组为42%，第5组为33%。与第3、4和5组相比，第2组细菌移位显著增加（P = 0.001）。第2组的细菌计数（CFU/g）显著高于第3、4和5组（P = 0.001）。回肠标本的组织病理学检查显示，与假手术组相比，第2 - 5组绒毛高度显著降低（P = 0.001）。第3组和第5组的平均绒毛高度显著高于第4组（P = 0.001）。
结论
发现布拉酵母菌在成功控制细菌移位和改善肠道屏障功能方面有效。
Zotero 插件