小肠结肠炎耶尔森菌1A生物变种β-内酰胺酶基因blaA和blaB的分子特征
Molecular characterization of beta-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A.
作者信息
Sharma Sachin, Mittal Shilpi, Mallik Sarita, Virdi Jugsharan S
机构信息
Microbial Pathogenicity Laboratory, Department of Microbiology, University of Delhi South Campus, New Delhi, India.
出版信息
FEMS Microbiol Lett. 2006 Apr;257(2):319-27. doi: 10.1111/j.1574-6968.2006.00191.x.
The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA but not in blaB. Cluster analysis of blaA restriction profiles grouped the strains into three groups. The blaA gene of Y. enterocolitica biovar 1A showed a high degree of sequence homology to that of Y. enterocolitica 8081 (biovar 1B) and Y. enterocolitica Y-56 (biovar 4), whereas homology was low with class A beta-lactamase genes of other members of the family Enterobacteriaceae. The pI 8.7 of enzyme Bla-A of Y. enterocolitica biovar 1A was similar to that of biovars 2, 3 and 4. The enzyme Bla-B focused at 6.8 and 7.1, indicating that biovar 1A strains produced a 'B-like' enzyme. This is the first study to have investigated the genetic heterogeneity of the beta-lactamase genes of Y. enterocolitica.
通过PCR扩增，在从印度、德国、法国和美国分离出的小肠结肠炎耶尔森菌1A生物变种菌株中检测到β-内酰胺酶基因blaA和blaB。在所有菌株中均检测到这两个基因。聚合酶链反应-限制性片段长度多态性分析显示blaA存在基因异质性，而blaB没有。blaA限制性图谱的聚类分析将菌株分为三组。小肠结肠炎耶尔森菌1A生物变种的blaA基因与小肠结肠炎耶尔森菌8081（1B生物变种）和小肠结肠炎耶尔森菌Y-56（4生物变种）的blaA基因具有高度序列同源性，而与肠杆菌科其他成员的A类β-内酰胺酶基因同源性较低。小肠结肠炎耶尔森菌1A生物变种的Bla-A酶的pI 8.7与2、3和4生物变种的相似。Bla-B酶聚焦于6.8和7.1，表明1A生物变种菌株产生一种“B类”酶。这是首次对小肠结肠炎耶尔森菌β-内酰胺酶基因的遗传异质性进行研究。
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