钌（II）的两种混合配体配合物的合成、表征及DNA结合研究。
Synthesis, characterization and DNA binding studies of two mixed ligand complexes of ruthenium(II).
作者信息
Lawrence D, Vaidyanathan V G, Nair Balachandran Unni
机构信息
Chemical Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, Tamil Nadu, India.
出版信息
J Inorg Biochem. 2006 Jul;100(7):1244-51. doi: 10.1016/j.jinorgbio.2006.02.003. Epub 2006 Mar 22.
Two mixed ligand complexes [Ru(bpy)(2)(DMHBT)]Cl(2)(1) and [Ru(phen)(2)(DMHBT)]Cl(2) (2) (where DMHBT is 11,13-dimethyl-13H-4,5,9,11,14-hexaaza-benzo[b]triphenylene-10,12-dione) have been synthesized and characterized by electrospray ionization (ESI) mass, (1)H-(1)H correlation spectroscopy (COSY), electronic spectroscopy, fluorescence spectroscopy and cyclic voltammetry. Spectroscopic titration and viscosity changes of calf thymus (CT)-DNA in the presence of incremental amount of complexes 1 and 2 clearly demonstrate that both these complexes bind intercalatively to DNA, with binding constant 2.87+/-0.20 x 10(4)M(-1) and 1.01+/-0.20 x 10(5)M(-1) for complexes 1 and 2, respectively. All the experimental evidences suggest that the ancillary ligand 2,2'-bipyridine (bpy) and 1,10-phenanthroline (phen) influences the intercalative binding of these complexes to DNA.
合成了两种混合配体配合物[Ru(bpy)(2)(DMHBT)]Cl(2)(1)和[Ru(phen)(2)(DMHBT)]Cl(2)(2)(其中DMHBT为11,13 - 二甲基 - 13H - 4,5,9,11,14 - 六氮杂 - 苯并[b]三苯撑 - 10,12 - 二酮)，并通过电喷雾电离(ESI)质谱、(1)H-(1)H相关光谱(COSY)、电子光谱、荧光光谱和循环伏安法对其进行了表征。在配合物1和2的量逐渐增加的情况下，对小牛胸腺(CT)-DNA进行光谱滴定和粘度变化分析，结果清楚地表明这两种配合物均以插入方式与DNA结合，配合物1和2的结合常数分别为2.87±0.20×10(4)M(-1)和1.01±0.20×10(5)M(-1)。所有实验证据表明，辅助配体2,2'-联吡啶(bpy)和1,10 - 菲咯啉(phen)会影响这些配合物与DNA的插入结合。
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