金表面光交联小分子阵列的表面等离子体共振成像

SPR imaging of photo-cross-linked small-molecule arrays on gold.

作者信息

Kanoh Naoki, Kyo Motoki, Inamori Kazuki, Ando Ami, Asami Aya, Nakao Aiko, Osada Hiroyuki

机构信息

Antibiotics Laboratory and Beam Application Team, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

出版信息

Anal Chem. 2006 Apr 1;78(7):2226-30. doi: 10.1021/ac051777j.

DOI:10.1021/ac051777j

PMID:16579601

Abstract

Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor alpha were observed using this platform.

摘要

鉴定目标蛋白的小分子配体有助于分析该蛋白在生物系统中的功能。小分子微阵列能够以高通量方式快速检测此类配体-蛋白相互作用，不过需要对蛋白进行标记才能观察到这些相互作用。通过将表面等离子体共振（SPR）成像技术与我们最近开发的光交联小分子阵列平台相结合，我们开发了一种新型平台，该平台能够原位观察金表面的光交联小分子与溶液中未标记蛋白之间的相互作用。利用该平台观察了雌激素和雄激素物质与雌激素受体α的相互作用。