人滋养层细胞在体外可代谢25-羟基维生素D3。

Human trophoblastic cells metabolize 25-hydroxyvitamin D3 in vitro.

作者信息

Okabe T, Ishizaka S, Fujisawa M

机构信息

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Jpn J Med. 1991 Jul-Aug;30(4):326-9. doi: 10.2169/internalmedicine1962.30.326.

DOI:10.2169/internalmedicine1962.30.326

PMID:1658420

Abstract

Human trophoblastic cells (T3M-3) metabolized 25-hydroxyvitamin D3 into two more polar metabolites during in vitro incubations. A two-step high pressure liquid chromatography system revealed two unique elution positions of those trophoblastic cell-derived metabolites that exactly co-migrated with the elution positions of 5(Z)-19-nor-10-oxo-25-hydroxyvitamin D3 and 5(E)-19-nor-10-oxo-25-hydroxyvitamin D3, respectively. These unique metabolites did not bind specifically to a protein receptor for 1,25-dihydroxyvitamin D3.

摘要

人滋养层细胞（T3M-3）在体外培养过程中将25-羟基维生素D3代谢为两种极性更强的代谢产物。两步高压液相色谱系统显示，这些滋养层细胞衍生的代谢产物有两个独特的洗脱位置，分别与5(Z)-19-去甲-10-氧代-25-羟基维生素D3和5(E)-19-去甲-10-氧代-25-羟基维生素D3的洗脱位置完全共迁移。这些独特的代谢产物不与1,25-二羟基维生素D3的蛋白质受体特异性结合。