烟草叶绿体 tRNA(UUU)基因含有 2.5 千碱基对的内含子：内含子中的开放阅读框和保守边界序列。
Tobacco chloroplast tRNA(UUU) gene contains a 2.5-kilobase-pair intron: An open reading frame and a conserved boundary sequence in the intron.
机构信息
Department of Botany, Hokkaido University, Sapporo 060, Japan.
出版信息
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3557-61. doi: 10.1073/pnas.82.11.3557.
The nucleotide sequence of a tRNA(Lys)(UUU) gene on tobacco (Nicotiana tabacum) chloroplast DNA has been determined. This gene is located 215 base pairs upstream from the gene for the 32,000-dalton thylakoid membrane protein on the same DNA strand and has a 2526-base-pair intron in the anticodon loop. The intron boundary sequence does not follow the G-U/A-G rule but is similar to those of tobacco chloroplast split genes for tRNA(Gly)(UCC) and ribosomal proteins L2 and S12. The intron contains one major open reading frame of 509 codons. The codon usage in the open reading frame resembles those observed in the genes for tobacco chloroplast proteins so far analyzed. The primary transcript of this tRNA gene is 2.7 kilobases long.
已测定烟草（Nicotiana tabacum）叶绿体 DNA 上 tRNA（Lys）（UUU）基因的核苷酸序列。该基因位于同一 DNA 链上编码 32000 道尔顿类囊体膜蛋白的基因上游 215 个碱基对，反密码环中有一个 2526 个碱基对的内含子。内含子边界序列不符合 G-U/A-G 规则，但与烟草叶绿体 tRNA（Gly）（UCC）和核糖体蛋白 L2 和 S12 的分裂基因相似。内含子包含一个 509 个密码子的主要开放阅读框。开放阅读框中的密码子使用情况与迄今为止分析的烟草叶绿体蛋白基因中观察到的情况相似。该 tRNA 基因的初级转录物长 2.7 千碱基。
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