体内人类自然杀伤细胞分化不同阶段的证据。
Evidence for discrete stages of human natural killer cell differentiation in vivo.
作者信息
Freud Aharon G, Yokohama Akihiko, Becknell Brian, Lee Melissa T, Mao Hsiaoyin C, Ferketich Amy K, Caligiuri Michael A
机构信息
Medical Scientist Program, The Ohio State University, Columbus, OH 43210, USA.
出版信息
J Exp Med. 2006 Apr 17;203(4):1033-43. doi: 10.1084/jem.20052507. Epub 2006 Apr 10.
Human natural killer (NK) cells originate from CD34(+) hematopoietic progenitor cells, but the discrete stages of NK cell differentiation in vivo have not been elucidated. We identify and functionally characterize, from human lymph nodes and tonsils, four NK cell developmental intermediates spanning the continuum of differentiation from a CD34(+) NK cell progenitor to a functionally mature NK cell. Analyses of each intermediate stage for CD34, CD117, and CD94 cell surface expression, lineage differentiation potentials, capacity for cytokine production and natural cytotoxicity, and ETS-1, GATA-3, and T-BET expression provide evidence for a new model of human NK cell differentiation in secondary lymphoid tissues.
人类自然杀伤（NK）细胞起源于CD34(+)造血祖细胞，但NK细胞在体内分化的离散阶段尚未阐明。我们从人淋巴结和扁桃体中鉴定出并对四种NK细胞发育中间体进行了功能表征，它们跨越了从CD34(+)NK细胞祖细胞到功能成熟NK细胞的连续分化过程。对每个中间阶段的CD34、CD117和CD94细胞表面表达、谱系分化潜能、细胞因子产生和自然细胞毒性能力以及ETS-1、GATA-3和T-BET表达进行分析，为次级淋巴组织中人类NK细胞分化的新模型提供了证据。
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