妊娠剧吐中腺苷与辅助性T细胞1/辅助性T细胞2平衡的关系。

Relationship between adenosine and T-helper 1/T-helper 2 balance in hyperemesis gravidarum.

作者信息

Kiyokawa Yasuko, Yoneyama Yoshio

机构信息

Department of Reproductive Medicine, Tokyo Institute of Women's Health, 2-5-9, Honkomagome, Tokyo 113-0021, Japan.

出版信息

Clin Chim Acta. 2006 Aug;370(1-2):137-42. doi: 10.1016/j.cca.2006.02.001. Epub 2006 Feb 28.

DOI:10.1016/j.cca.2006.02.001

PMID:16616909

Abstract

BACKGROUND

Adenosine modulates the T-helper (Th) 1/T-helper (Th) 2 balance. We evaluated the relationship between changes in plasma adenosine and the T-helper (Th) 1/T-helper (Th) 2 balance in hyperemesis gravidarum.

METHODS

Plasma adenosine concentrations and the Th1/Th2 ratio were examined in the peripheral blood of 24 women with hyperemesis gravidarum and normal pregnancies. The proportion of CD4-positive cells that expressed intracellular cytokines (interferon gamma and interleukin 4) was analyzed by flow cytometry. The ratio of interferon-gamma-secreting cells to interleukin-4-secreting cells was taken as the Th1/Th2 ratio in vivo. The change induced by adenosine-receptor blocker 8-sulfophenyltheophylline was also measured in vitro to evaluate the possible role of adenosine in modifying the Th1/Th2 balance.

RESULTS

In hyperemesis gravidarum, plasma adenosine and the proportion of interleukin-4-secreting cells were increased significantly, and the Th1/Th2 ratio was significantly lower than in normal pregnancy (p<0.05). The decrease in the proportion of IL-4-secreting cells after adenosine receptor blockade in hyperemesis gravidarum significantly exceeded that of normal pregnancy (p<0.05).

CONCLUSIONS

Increased plasma adenosine may be involved in regulating the Th1/Th2 balance in hyperemesis gravidarum.

摘要

背景

腺苷可调节辅助性T细胞（Th）1/辅助性T细胞（Th）2平衡。我们评估了妊娠剧吐患者血浆腺苷变化与辅助性T细胞（Th）1/辅助性T细胞（Th）2平衡之间的关系。

方法

检测了24例妊娠剧吐患者和正常妊娠女性外周血中的血浆腺苷浓度及Th1/Th2比值。通过流式细胞术分析表达细胞内细胞因子（干扰素γ和白细胞介素4）的CD4阳性细胞比例。将分泌干扰素γ的细胞与分泌白细胞介素4的细胞的比值作为体内Th1/Th2比值。还在体外测量了腺苷受体阻滞剂8-磺基苯基茶碱诱导的变化，以评估腺苷在调节Th1/Th2平衡中的可能作用。