Department of Botany, University of Edinburgh, Edinburgh EH9 3JH, Scotland.
Plant Physiol. 1978 Dec;62(6):975-7. doi: 10.1104/pp.62.6.975.
Triton X-100 solubilized chloroplast DNA but not nuclear DNA from a mixture of chloroplasts and nuclei. The buoyant density of chloroplast DNA was different from that of the satellite DNA in all of the species examined (Phaseolus coccineus, Cucumis sativus, Cucumis melo, Antirrhinum majus, Vicia faba, Oenothera fruiticosa youngii). Chloroplast DNA constituted between 4.3% and 0.25% of the total leaf DNA in these species, and was present as 5 to 20 copies in each chloroplast.
Triton X-100 可使叶绿体 DNA 而不是叶绿体和细胞核混合物中的核 DNA 溶解。在所研究的所有物种(菜豆、黄瓜、甜瓜、金鱼草、蚕豆、月见草)中,叶绿体 DNA 的浮力密度均不同于卫星 DNA。叶绿体 DNA 占这些物种总叶 DNA 的 4.3%至 0.25%,每个叶绿体中存在 5 至 20 个拷贝。