Department of Pomology, University of California, Davis, California 95616.
Plant Physiol. 1980 May;65(5):1014-6. doi: 10.1104/pp.65.5.1014.
Mature ;Bartlett' pear (Pyrus communis) fruits were ripened at 20 C. Fruits at different stages of ripeness were homogenized, and extracts of the low speed pellet (crude cell wall) were prepared. These extracts contained polygalacturonase, pectin esterase, and activity against seven p-nitrophenyl glycoside substrates. Polygalacturonase, alpha-galactosidase, and alpha-mannosidase increased in activity as the fruit ripened. Cellulase and activities against pear wall xylan and arabinan were absent from the extracts.
成熟的巴旦梨树(Pyrus communis)果实于 20°C 下成熟。将处于不同成熟阶段的果实匀浆,提取低速沉淀(粗细胞壁)的提取物。这些提取物含有多聚半乳糖醛酸酶、果胶酯酶和对七种对硝基苯糖苷底物的活性。随着果实成熟,多聚半乳糖醛酸酶、α-半乳糖苷酶和α-甘露糖苷酶的活性增加。纤维素酶和对梨壁木聚糖和阿拉伯聚糖的活性不存在于提取物中。