Department of Botany, University of Massachusetts, Amherst, Massachusetts 01003.
Plant Physiol. 1984 Oct;76(2):386-91. doi: 10.1104/pp.76.2.386.
Electron transport activity at the cell surface of intact oat seedlings (Avena sativa L. cv Garry) was examined by measuring the oxidation and/or reduction of agents in the medium bathing the roots. Oxidation of NADH with or without added electron acceptors and reduction of ferricyanide by an endogenous electron donor were detected. The activities appear to be due to electron transfer at, or across, the plasma membrane and not due to reagent uptake or leakage of oxidants or reductants. NADH-ferricyanide oxidoreductase activity was also detected in plasma membrane-enriched preparations from Avena roots. Based on redox responses to pH, various ions, and to a variety of electron donors and acceptors, the results indicate that more than one electron transport system is present at the plasma membrane.
通过测量浸泡在根系中的介质中的试剂的氧化和/或还原来检测完整燕麦幼苗(Avena sativa L. cv Garry)细胞表面的电子传递活性。检测了添加电子受体的 NADH 的氧化和/或不添加电子受体的 NADH 的氧化以及内源性电子供体对铁氰化物的还原。这些活性似乎是由于在质膜上或穿过质膜进行电子转移引起的,而不是由于试剂摄取或氧化剂或还原剂的渗漏引起的。在燕麦根的富含质膜的制剂中也检测到 NADH-铁氰化物氧化还原酶活性。基于对 pH、各种离子以及各种电子供体和受体的氧化还原反应,结果表明质膜上存在不止一种电子传递系统。