豌豆叶绿体中磷脂酸合成的最后一步发生在内包膜膜上。
Final step of phosphatidic Acid synthesis in pea chloroplasts occurs in the inner envelope membrane.
作者信息
Andrews J, Ohlrogge J B, Keegstra K
机构信息
Botany Department, University of Wisconsin, Madison, Wisconsin 53706.
出版信息
Plant Physiol. 1985 Jul;78(3):459-65. doi: 10.1104/pp.78.3.459.
Abstract
The second enzyme of phosphatidic acid synthesis from glycerol-3-phosphate, 1-acylglycerophospate acyltransferase, was localized to the inner envelope membrane of pea chloroplasts. The activity of this enzyme was measured by both a coupled enzyme assay and a direct enzyme assay. Using the coupled enzyme assay, phosphatidic acid phosphatase was also localized to the inner envelope membrane, although this enzyme has very low activity in pea chloroplasts. The addition of UDP-galactose to unfractionated pea chloroplast envelope preparations did not result in significant conversion of newly synthesized diacylglycerol to monogalactosyldiacylglycerol. Thus, the envelope synthesized phosphatidic acid may not be involved in galactolipid synthesis in pea chloroplasts.
摘要
从3-磷酸甘油合成磷脂酸的第二种酶，即1-酰基甘油磷酸酰基转移酶，定位于豌豆叶绿体的内膜。该酶的活性通过偶联酶法和直接酶法进行测定。使用偶联酶法时，磷脂酸磷酸酶也定位于内膜，尽管该酶在豌豆叶绿体中的活性非常低。向未分级的豌豆叶绿体被膜制剂中添加UDP-半乳糖，并不会使新合成的二酰基甘油大量转化为单半乳糖基二酰基甘油。因此，被膜合成的磷脂酸可能不参与豌豆叶绿体中半乳糖脂的合成。
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