大鼠大脑、小脑和下丘脑的乙酰胆碱酯酶活性差异

Differential acetylcholinesterase activity in rat cerebrum, cerebellum and hypothalamus.

作者信息

Roy Rini, Chaudhuri Aditi Nag

机构信息

Department of Microbiology, Lady Brabourne College, P1/2 Suhrawardy Avenue, Kolkata 700 017, India.

出版信息

Indian J Exp Biol. 2006 May;44(5):381-6.

PMID:16708891

Abstract

Acetylcholinesterase (AChE) has been purified from three different regions of rat brain using Sephadex G 200 column. SDS PAGE (6%) showed single band for the purified AChE fractions. Purified and lyophilized AChE from different (NH4)2SO4 precipitated fractions of three brain parts were utilized for in vitro enzyme kinetics using Dimethoate (Dmt) as inhibitor. K(m) values for cerebellum and hypothalamus were almost similar whereas cerebrum showed a different K(m) value compared to other two regions. With the drug Rivastigmine it was found that % G1 and G4 forms of AChE in three different parts of brain are different.

摘要

已使用葡聚糖G 200柱从大鼠脑的三个不同区域纯化了乙酰胆碱酯酶（AChE）。SDS-PAGE（6%）显示纯化的AChE组分呈单一带。将来自三个脑区不同硫酸铵沉淀组分的纯化并冻干的AChE用于以乐果（Dmt）作为抑制剂的体外酶动力学研究。小脑和下丘脑的米氏常数（K(m)）值几乎相似，而大脑与其他两个区域相比显示出不同的K(m)值。使用药物卡巴拉汀发现，大脑三个不同部位的AChE的G1和G4形式的百分比不同。