频域近红外光谱技术在脑氧合评估中的应用：一项在活体和尸体上的验证研究
Frequency domain near-infrared spectroscopy technique in the assessment of brain oxygenation: a validation study in live subjects and cadavers.
作者信息
Gatto Rodolfo, Hoffman William, Mueller Mark, Flores Arthur, Valyi-Nagy Tibor, Charbel Fady T
机构信息
Department of Neurosurgery, University of Illinois at Chicago, Chicago, IL 60612, USA.
出版信息
J Neurosci Methods. 2006 Oct 30;157(2):274-7. doi: 10.1016/j.jneumeth.2006.04.013. Epub 2006 May 24.
BACKGROUND
Studies with continuous wave near infrared spectroscopy (CW-NIRS) have shown little difference in brain oxygenation of dead compared to live subjects. We determined brain oxyhemoglobin (OHb) and deoxyhemoglobin (HHb) concentrations in healthy volunteers and cadavers using frequency domain near infrared spectroscopy (FD-NIRS).
METHODS
Regional OHb and HHb, brain oxygen saturation (SO2), and total hemoglobin (tHb) were determined. Nine patients who died in the hospital were evaluated by FD-NIRS in the morgue 7-96 h after death was confirmed. Ten volunteers served as a control group.
RESULTS
Absolute concentrations of brain tissue OHb and HHb were 24.9 +/- 9.1 uM and 13.8 +/- 32 uM, respectively, in live subjects. In dead subjects, OHb was 1.3 +/- 2.1 uM and HHb was 30.8 +/- 14.4 uM (both P < 0.05 compared to live). OHb showed a 90% decrease within 7h of death. There was a significant trend for a continued decrease in OHb from 7 to 96 h.
CONCLUSION
OHb decreased and HHb increased in dead patients compared to live volunteers. Depletion of OHb primarily occurred within 7 h of death but continued gradually over 96 h. FD-NIRS was a novel technique for determining OHb and HHb changes following death.
背景
连续波近红外光谱（CW-NIRS）研究表明，死亡受试者与活体受试者的脑氧合情况差异不大。我们使用频域近红外光谱（FD-NIRS）测定了健康志愿者和尸体中的脑氧合血红蛋白（OHb）和脱氧血红蛋白（HHb）浓度。
方法
测定局部OHb和HHb、脑氧饱和度（SO2）和总血红蛋白（tHb）。9例在医院死亡的患者在确认死亡后7-96小时于停尸房接受FD-NIRS评估。10名志愿者作为对照组。
结果
活体受试者脑组织OHb和HHb的绝对浓度分别为24.9±9.1μM和13.8±32μM。在死亡受试者中，OHb为1.3±2.1μM，HHb为30.8±14.4μM（与活体相比，两者P<0.05）。OHb在死亡后7小时内下降了90%。从7小时到96小时，OHb持续下降有显著趋势。
结论
与活体志愿者相比，死亡患者的OHb降低，HHb升高。OHb的消耗主要发生在死亡后7小时内，但在96小时内逐渐持续。FD-NIRS是一种测定死亡后脑组织OHb和HHb变化的新技术。
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