长期降低家兔主动脉血流量对脊髓灰质的影响。还原型辅酶Ⅱ黄递酶的组织化学研究。

The effect of long-term reduction of aortic blood flow on spinal cord gray matter in the rabbit. Histochemical study of NADPH-diaphorase.

作者信息

Kluchova Darina, Kloc Peter, Klimcik Roman, Molcakova Adriana, Lovasova Kvetuse

机构信息

Department of Anatomy, University of P. J. Safarik, Srobarova 2, Kosice, 040 01, Slovak Republic.

出版信息

Cell Mol Neurobiol. 2006 Oct-Nov;26(7-8):1253-64. doi: 10.1007/s10571-006-9068-2. Epub 2006 May 30.

DOI:10.1007/s10571-006-9068-2

PMID:16733796

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11520633/

Abstract

The aim of this work was to study the influence of reduced aortic blood flow on NADPH-diaphorase (NADPH-d) staining in the gray matter of L4-S3 spinal cord segments. 2. Surgery was performed on the abdominal aorta of the rabbit. Spinal cord ischemia was introduced by infrarenal aortic constriction to 30% from the normal blood flow. Animals were allowed to survive 1 week, 1 month and 3 months after surgery. Neurological outcome was studied in relation to the duration of aortic occlusion. The NADPH-d histochemistry was used for the visualisation of spinal cord sections. 3. The most affected area of the spinal cord was pericentral region, and slight changes were seen in the NADPH-d activities of both dorsal and ventral horns. One week after surgery, NADPH-d positive pericentral neurons were almost unchanged in their shape and intensity of staining, the only difference was seen in slightly increased staining of the background around the central canal. One month following surgery neurons exhibited shrinkage or were swollen, NADPH-d staining was less intensive in the pericentral zone and positively stained vessels were present. 4. Three months of ischemia influenced the NADPH-d activity: (a) In the pericentral region were seen intensively NADPH-d stained neurons almost normal in shape of their bodies but with shortened processes or without them; (b) NADPH-d staining of neuropil was greatly enhanced mostly around the central canal and in the dorsal commissure; (c) Numerous vessels were present in the pericentral zone and in the location of the ventral horn. 5. It can be concluded that the reduction of blood flow in the abdominal aorta makes most changes in the pericentral region of the rabbit spinal cord. Increased NADPH-d staining of neuropil and the presence of positively stained vessels reflect increased NADPH-d/NOS production in the spinal cord gray matter after long-term incomplete aortic occlusion.

摘要

本研究的目的是探讨主动脉血流减少对L4 - S3脊髓节段灰质中还原型辅酶Ⅱ黄递酶（NADPH-d）染色的影响。2. 对兔腹主动脉进行手术。通过肾下腹主动脉缩窄使脊髓缺血，血流量降至正常的30%。术后让动物存活1周、1个月和3个月。研究神经功能结果与主动脉阻断时间的关系。采用NADPH-d组织化学方法对脊髓切片进行观察。3. 脊髓受影响最严重的区域是中央周围区域，背角和腹角的NADPH-d活性均有轻微变化。术后1周，中央周围NADPH-d阳性神经元的形态和染色强度几乎未变，唯一的差异是中央管周围背景染色略有增加。术后1个月，神经元出现萎缩或肿胀，中央周围区域的NADPH-d染色强度降低，且出现阳性染色的血管。4. 缺血3个月影响了NADPH-d活性：（a）在中央周围区域可见NADPH-d染色强烈的神经元，其胞体形态基本正常，但突起缩短或无突起；（b）神经毡的NADPH-d染色大多在中央管周围和背侧连合处显著增强；（c）中央周围区域和腹角部位有大量血管。5. 可以得出结论，腹主动脉血流减少使兔脊髓中央周围区域发生了大部分变化。神经毡NADPH-d染色增加以及阳性染色血管的出现反映了长期不完全主动脉阻断后脊髓灰质中NADPH-d/一氧化氮合酶（NOS）产生增加。