大鼠体内β-半乳糖苷酶的特异性及多种形式
Specificity and multiple forms of beta-galactosidase in the rat.
作者信息
Furth A J, Robinson D
机构信息
Department of Nutrition, Queen Elizabeth College, London, W. 8.
出版信息
Biochem J. 1965 Oct;97(1):59-66. doi: 10.1042/bj0970059.
Abstract
- Ten rat tissues and organs have been assayed for beta-galactosidase with phenyl beta-d-galactoside, p-nitrophenyl beta-d-galactoside, p-aminophenyl beta-d-galactoside and 4-methylumbelliferyl beta-d-galactoside as substrates. 2. The relative activities of these tissues are independent of the mode of assay, and maximum rates of hydrolysis are not greatly affected by the nature of the substrate. 3. Inhibition studies suggest the liver enzyme has no associated beta-glucosidase activity. 4. There is no cellular localization of preferential activity towards any of the four substrates in liver, kidney or spleen. 5. Evidence suggesting the non-destructive penetration of liver lysosomal membranes by p-nitrophenyl beta-d-galactoside is presented. 6. Liver lysosomal beta-galactosidase exists in multiple forms that can be separated on DEAE-cellulose, and the enzyme components that are bound to the membrane appear to be similar to those of the lysosome sap. 7. The chromatographic pattern of enzyme excreted in the urine is compared with those from the kidney, intestine, spleen and liver.
摘要
- 以苯基β-D-半乳糖苷、对硝基苯基β-D-半乳糖苷、对氨基苯基β-D-半乳糖苷和4-甲基伞形酮基β-D-半乳糖苷为底物，对十只大鼠的组织和器官进行了β-半乳糖苷酶检测。2. 这些组织的相对活性与检测方式无关，水解的最大速率也不受底物性质的很大影响。3. 抑制研究表明，肝脏酶没有相关的β-葡萄糖苷酶活性。4. 在肝脏、肾脏或脾脏中，对四种底物中的任何一种都没有优先活性的细胞定位。5. 提出了对硝基苯基β-D-半乳糖苷能无损穿透肝脏溶酶体膜的证据。6. 肝脏溶酶体β-半乳糖苷酶以多种形式存在，可在DEAE-纤维素上分离，与溶酶体液结合的酶成分似乎与溶酶体液中的相似。7. 将尿液中排泄的酶的色谱图谱与肾脏、肠道、脾脏和肝脏中的色谱图谱进行了比较。
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