Li Pei, Ling Zhi-qiang, Yang Hong-yan, Huang You-tian, Zhao Ji-min, Zhao Ming-yao, Dong Zi-ming
Department of Pathophysiology, School for Basic Medical Sciences of Zhengzhou University, Zhengzhou 450052, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 May;26(5):632-4.
To investigate the differentially expressed genes between human esophageal squamous cell carcinoma (ESCC) and normal esophageal mucosa and explore an effective method with high throughput for screening the molecular markers closely correlated with the development, invasion and metastasis of ESCC.
With cDNA microarray and laser capture microdissection, T7-based amplification were used to detect the mRNA from both the primary carcinoma and the corresponding esophageal epithelium in 15 ESCC cases, and the results were analyzed by bioinformatics methods.
Among the 886 target genes, 110 (12.42%) genes were differentially expressed commonly at least twice in all the 15 samples, including 56 (6.32%) up-regulated by at least 2 folds and 54 (6.09%) down-regulated by at least 0.5 folds.
Many ESCC-associated genes were screened by the high-throughput gene chip method, and functional study of these genes may help to identify the key genes or pathways involved in the pathogenesis and development of ESCC.
研究人食管鳞状细胞癌（ESCC）与正常食管黏膜之间的差异表达基因，探索一种高通量的有效方法以筛选与ESCC发生、侵袭和转移密切相关的分子标志物。
采用cDNA微阵列和激光捕获显微切割技术，运用基于T7的扩增方法检测15例ESCC患者原发癌组织及相应食管上皮组织的mRNA，并通过生物信息学方法对结果进行分析。
在886个靶基因中，110个（12.42%）基因在所有15个样本中均至少有两次差异表达，其中56个（6.32%）基因上调至少2倍，54个（6.09%）基因下调至少0.5倍。
通过高通量基因芯片方法筛选出了许多与ESCC相关的基因，对这些基因进行功能研究可能有助于确定参与ESCC发病机制和发展的关键基因或通路。
