胃电刺激对大鼠腹内侧下丘脑（VMH）中胃扩张反应性神经元的兴奋作用。

Excitatory effects of gastric electrical stimulation on gastric distension responsive neurons in ventromedial hypothalamus (VMH) in rats.

作者信息

Sun Xiangrong, Tang Ming, Zhang Jing, Chen Jiande D Z

机构信息

Department of Pathophysiology, Medical College of Qingdao University, China.

出版信息

Neurosci Res. 2006 Aug;55(4):451-7. doi: 10.1016/j.neures.2006.05.001.

DOI:10.1016/j.neures.2006.05.001

PMID:16766073

Abstract

INTRODUCTION

Gastric electrical stimulation (GES) has been used for the treatment of obesity with unclear central mechanisms. The purpose of this study was to investigate the effects of GES on the neuronal activity in the ventromedial hypothalamus (VMH).

METHODS

Extracellular potentials of single neurons in VMH were recorded in 52 anesthetized rats. Neurons were classified as gastric distension-excitatory (GD-E) neurons or GD-inhibitory (GD-I) neurons. GES with four sets of parameters was applied for comparison.

RESULTS

Eighty two neurons out of 96 (85.41%) in VMH responded to gastric distension (GD). 37.8% were GD-E neurons and 51(62.2%) were GD-I neurons. 55.0%, 17.6%, 77.8%, 14.3% of GD-E neurons were excited by four sets of parameters: GES1 (standard), GES2 (reduced pulse numbers), GES3 (increased pulse width) and GES4 (reduced frequency), respectively. More GD-E neurons were excited by GES3 (P < 0.05 versus GES2 or GES4) and by GES1 (P < 0.02 versus GES2 or GES4). Among the GD-I neurons, 63.6, 37.9, 73.3, and 51.9% neurons were excited by GES1-4, respectively.

CONCLUSION

GES with parameters used for treating obesity excites GD-responsive neurons in VMH. The excitatory effect of GES is related to the strength of stimulation, including pulse frequency and width as well as pulse train on-time.

摘要

引言

胃电刺激（GES）已被用于治疗肥胖症，但其中枢机制尚不清楚。本研究的目的是探讨GES对腹内侧下丘脑（VMH）神经元活动的影响。

方法

在52只麻醉大鼠中记录VMH中单个神经元的细胞外电位。神经元被分为胃扩张兴奋性（GD-E）神经元或胃扩张抑制性（GD-I）神经元。应用四组参数的GES进行比较。

结果

VMH中96个神经元中有82个（85.41%）对胃扩张（GD）有反应。37.8%为GD-E神经元，51个（62.2%）为GD-I神经元。四组参数的GES分别使55.0%、17.6%、77.8%、14.3%的GD-E神经元兴奋：GES1（标准）、GES2（减少脉冲数）、GES3（增加脉冲宽度）和GES4（降低频率）。GES3使更多的GD-E神经元兴奋（与GES2或GES4相比，P<0.05），GES1也使更多的GD-E神经元兴奋（与GES2或GES4相比，P<0.02）。在GD-I神经元中，GES1-4分别使63.6%、37.9%、73.3%和51.9%的神经元兴奋。