Esteban A, Abarca M L, Bragulat M R, Cabañes F J
Grup de Micologia Veterinària, Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Barcelona, Spain.
Food Addit Contam. 2006 Jun;23(6):616-22. doi: 10.1080/02652030600599124.
The effect of pH (2-10) on growth and ochratoxin A (OTA) production by 12 Aspergillus niger aggregate strains was studied in two culture media: Czapek yeast autolysate agar (CYA) and yeast extract sucrose agar (YES), over 30 days. The strains were selected to include different sources, different reported abilities to produce OTA and different ITS-5.8S rDNA RFLP patterns. YES was a better culture medium than CYA for OTA production. In this medium, OTA was produced from pH 2 or 3 to 10 depending on the strain. The results show the ability of A. niger aggregate strains not only to grow, but also to produce OTA over a wide pH range. The results will lead to a better understanding of the role of A. niger aggregate strains in the OTA contamination of several food commodities.
在两种培养基(察氏酵母自溶物琼脂(CYA)和酵母提取物蔗糖琼脂(YES))中,研究了pH值(2 - 10)对12株黑曲霉聚集菌株生长及赭曲霉毒素A(OTA)产生的影响,培养时间为30天。这些菌株的选择涵盖了不同来源、不同报道的OTA产生能力以及不同的ITS - 5.8S rDNA RFLP模式。对于OTA的产生,YES培养基比CYA培养基更优。在该培养基中,根据菌株不同,OTA在pH值为2或3至10的范围内均可产生。结果表明黑曲霉聚集菌株不仅能够生长,而且能在较宽的pH范围内产生OTA。这些结果将有助于更好地理解黑曲霉聚集菌株在几种食品商品OTA污染中的作用。