[川崎病外周血T细胞中CD69、CD25和HLA-DR表达的变化]
[Changes in CD69, CD25 and HLA-DR expressions in peripheral blood T cells in Kawasaki disease].
作者信息
Zhang Yi-ying, Huang Xian-mei, Kang Man-li, Gong Fang-qi, Qian Bai-qin
机构信息
Department of Cardiology, Children's Hospital Affiliated to Medical College, Zhejiang University, Hangzhou 310003, China.
出版信息
Zhonghua Er Ke Za Zhi. 2006 May;44(5):329-32.
OBJECTIVE
The study was designed to investigate the changes in CD(69), CD(25) and HLA-DR expressions in peripheral blood T cell in Kawasaki disease (KD).
METHODS
The authors detected CD(69), CD(25) and HLA-DR expressions in peripheral blood T cell by using flow cytometry. The patients who met the diagnostic criteria for KD comprised sixteen boys and fifteen girls (4 - 60 months of age; mean, 26 +/- 18 months). All received intravenous gammaglobulin at a dose of 1 g/(kg.d), for 2 days and oral aspirin at a dose of 30 - 50 mg/(kg.d). In case of persistent fever, a repeated dose of intravenous gammaglobulin or I.V. methylprednisolone at a dose of 20 mg/(kg.d) for three daily doses was attempted. The authors tested blood samples from 17 healthy controls consisting of nine boys and eight girls (3 - 84 months of age; mean, 25 +/- 18 months) and the samples from 31 patients.
RESULTS
The percentage of peripheral blood CD(3)(+) T lymphocyte was (54.4 +/- 9.0)% in acute stage of KD and (65.0 +/- 7.0)% in healthy controls. There was a significant difference between the two groups (P < 0.001). The values of CD(69)(+) [(11.2 +/- 12.6)%, vs. (0.6 +/- 0.4)%], CD(25)(+) [(9.2 +/- 3.5)% vs. (3.9 +/- 1.8)%] and HLA-DR(+) [(8.3 +/- 5.0)% vs. (4.3 +/- 2.3)%] in KD patients were markedly increased compared to those of the healthy controls. After intravenous gammaglobulin treatment, the percentage of CD(3)(+)CD(69)(+) and CD(3)(+)CD(25)(+) significantly decreased [CD(3)(+)CD(69)(+): (14.0 +/- 13.0)% vs. (1.6 +/- 1.2)%, P < 0.05; CD(3)(+)CD(25)(+): (7.8 +/- 4.1)% vs. (2.0 +/- 0.6)%, P < 0.01]. However, the CD(3)(+) T lymphocytes increased [(50.8 +/- 5.0)% vs. (64.9 +/- 5.5)%, P < 0.01]. There was no significant difference in expression of CD(3)(+) T lymphocyte cell activating markers between coronary artery disease group and normal coronary artery group in KD cases (P > 0.05).
CONCLUSION
CD(3)(+) T cell activation in the early and middle stages is involved in the mechanism responsible for cardiovascular injury.
目的
本研究旨在调查川崎病（KD）患者外周血T细胞中CD(69)、CD(25)和HLA - DR表达的变化。
方法
作者采用流式细胞术检测外周血T细胞中CD(69)、CD(25)和HLA - DR的表达。符合KD诊断标准的患者包括16名男孩和15名女孩（4 - 60个月龄；平均26±18个月）。所有患者均接受剂量为1 g/(kg·d)的静脉注射丙种球蛋白治疗，持续2天，并接受剂量为30 - 50 mg/(kg·d)的口服阿司匹林治疗。若持续发热，则尝试重复静脉注射丙种球蛋白或静脉注射甲泼尼龙，剂量为20 mg/(kg·d)，每日3次。作者检测了17名健康对照者（9名男孩和8名女孩，3 - 84个月龄；平均25±18个月）的血样以及31例患者的血样。
结果
KD急性期外周血CD(3)(+) T淋巴细胞百分比为(54.4±9.0)%，健康对照者为(65.0±7.0)%。两组间差异有统计学意义（P < 0.001）。KD患者中CD(69)(+) [(11.2±12.6)%， vs. (0.6±0.4)%]、CD(25)(+) [(9.2±3.5)% vs. (3.9±1.8)%]和HLA - DR(+) [(8.3±5.0)% vs. (4.3±2.3)%]的值明显高于健康对照者。静脉注射丙种球蛋白治疗后，CD(3)(+)CD(69)(+)和CD(3)(+)CD(25)(+)的百分比显著降低[CD(3)(+)CD(69)(+): (14.0±13.0)% vs. (1.6±1.2)%，P < 0.05；CD(3)(+)CD(25)(+): (7.8±4.1)% vs. (2.0±0.6)%，P < 0.01]。然而，CD(3)(+) T淋巴细胞增加[(50.8±5.0)% vs. (64.9±5.5)%，P < 0.01]。KD病例中，冠状动脉疾病组与正常冠状动脉组之间CD(3)(+) T淋巴细胞活化标志物的表达无显著差异（P > 0.05）。
结论
早中期CD(3)(+) T细胞活化参与了心血管损伤的发病机制。
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