Bakir Mohammad Abdul, Sakamoto Mitsuo, Kitahara Maki, Matsumoto Mitsuharu, Benno Yoshimi
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Laboratory of Dairy Science and Technology, Kyodo Milk Industry Co. Ltd, Hinode, Tokyo 190-0182, Japan.
Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1639-1643. doi: 10.1099/ijs.0.64257-0.
Two Gram-negative, anaerobic, non-spore-forming rod-shaped organisms were isolated from human faeces. These isolates were tentatively identified as Bacteroides based on morphological and biochemical criteria and appeared closely related to Bacteroides vulgatus ATCC 8482(T). The 16S rRNA gene sequence analysis showed that the isolates were highly related to each other (99.5 %) and confirmed their placement in the genus Bacteroides. 16S rRNA gene sequence similarity values with close phylogenetic neighbours Bacteroides vulgatus ATCC 8482(T) (96 %) and Bacteroides massiliensis CCUG 48901(T) (93 %) preliminarily demonstrated that the organisms represented a novel species. The results of phenotypic, chemotaxonomic and 16S rRNA gene sequence analyses, and DNA-DNA homology values provided evidence that these two unknown isolates represent a single species and should be assigned to a novel species of the genus Bacteroides, as Bacteroides dorei sp. nov. The type strain is JCM 13471(T) (=DSM 17855(T)).
从人类粪便中分离出两种革兰氏阴性、厌氧、无芽孢的杆状菌。根据形态学和生化标准,这些分离株初步鉴定为拟杆菌属,且似乎与普通拟杆菌ATCC 8482(T)密切相关。16S rRNA基因序列分析表明,这些分离株彼此高度相关(99.5%),并证实它们属于拟杆菌属。与亲缘关系较近的普通拟杆菌ATCC 8482(T)(96%)和马赛拟杆菌CCUG 48901(T)(93%)的16S rRNA基因序列相似性值初步表明,这些菌代表一个新物种。表型、化学分类学和16S rRNA基因序列分析结果以及DNA-DNA同源性值提供了证据,表明这两个未知分离株代表一个单一物种,应归为拟杆菌属的一个新物种,即多雷拟杆菌(Bacteroides dorei sp. nov.)。模式菌株为JCM 13471(T)(=DSM 17855(T))。