Barr I G, McCaig M, Durrant C, Shaw R
WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Victoria 3052, Australia.
Vaccine. 2006 Nov 10;24(44-46):6675-8. doi: 10.1016/j.vaccine.2006.05.047. Epub 2006 Jun 8.
An ELISA assay was developed to allow the rapid and accurate identification of human influenza A N1 and N2 neuraminidases. Initial testing using a fetuin pre-coating of wells correctly identified 81.7% of the neuraminidase type from a series of human A(H1N1), A(H1N2) and A(H3N2) viruses. This result could be improved to detect the neuraminidase subtype of almost all human influenza A viruses from a large panel of viruses isolated from 2000 to 2005, if the fetuin pre-coating was removed and the viruses were coated directly onto wells. This method is simple, rapid and can be used to screen large numbers of currently circulating human influenza A viruses for their neurraminidase subtype and is a good alternative to RT-PCR.
开发了一种酶联免疫吸附测定（ELISA）方法，以实现对人甲型流感病毒N1和N2神经氨酸酶的快速准确鉴定。使用胎球蛋白预包被微孔板进行的初步测试，从一系列人A（H1N1）、A（H1N2）和A（H3N2）病毒中正确鉴定出了81.7%的神经氨酸酶类型。如果去除胎球蛋白预包被并将病毒直接包被在微孔板上，从2000年至2005年分离的大量病毒中检测几乎所有人甲型流感病毒的神经氨酸酶亚型，这一结果可以得到改善。该方法简单、快速，可用于筛选大量当前流行的人甲型流感病毒的神经氨酸酶亚型，是逆转录聚合酶链反应（RT-PCR）的良好替代方法。
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