[The genetic polymorphism of HLA-DQA1 and HLA-DQB1 genes of Chinese Han population in Jiangsu area is studied by PCR-sequence-based typing].

OBJECTIVE

To investigate the polymorphism of HLA-DQA1 and DQB1 genes of Han population in Jiangsu of China.

METHODS

The alleles and haplotypes frequencies of HLA-DQA1 and DQB1 genes in 100 unrelated healthy individuals were analyzed by using polymerase chain reaction-sequence-based typing (PCR-SBT).

RESULTS

Among the 7 DQA1 alleles detected, the most common allele was DQA10301/02/03 with a frequency of 29.5%, which was followed by DQA10501, DQA10102 and DQA10201 with frequencies of 18.5%, 17.0% and 12.5%, respectively. Of the 13 DQB1 alleles detected, DQB10201/02 allele (21.5%) was the most frequent allele, followed by DQB10301/09 (14.5%), DQB10303 (13.5%) and DQB10603 (11.5%). The most common DQA1 vs DQB1 haplotype was DQA10301/02/03 vs DQB10303 with a frequency of 12.5%, which was followed by the DQA10201-DQB10201/02 (10.5%),DQA10501-DQB10201/02 (9.5%) and DQA10501-DQB10301/09 (7.0%).

CONCLUSION

The distribution of HLA-DQ alleles and haplotypes in Jiangsu Han population shares some genetic characteristics with other population in northern of China, but has its own characteristics. The data will provide useful information for anthropology, organ transplantation and disease association studies.