Li Xi, Peng Yinghua, Qu Xiaogang
Division of Biological Inorganic Chemistry, Key Laboratory of Rare Earth Chemistry and Physics, Graduate School of the Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Nucleic Acids Res. 2006 Aug 2;34(13):3670-6. doi: 10.1093/nar/gkl513. Print 2006.
Single-walled carbon nanotubes (SWNTs) have been considered as the leading candidate for nanodevice applications ranging from gene therapy and novel drug delivery to membrane separations. The miniaturization of DNA-nanotube devices for biological applications requires fully understanding DNA-nanotube interaction mechanism. We report here, for the first time, that DNA destabilization and conformational transition induced by SWNTs are sequence-dependent. Contrasting changes for SWNTs binding to poly[dGdC]:poly[dGdC] and poly[dAdT]:poly[dAdT] were observed. For GC homopolymer, DNA melting temperature was decreased 40 degrees C by SWNTs but no change for AT-DNA. SWNTs can induce B-A transition for GC-DNA but AT-DNA resisted the transition. Our circular dichroism, competitive binding assay and triplex destabilization studies provide direct evidence that SWNTs induce DNA B-A transition in solution and they bind to the DNA major groove with GC preference.
单壁碳纳米管（SWNTs）被认为是纳米器件应用的主要候选材料，其应用范围涵盖从基因治疗、新型药物递送膜分离等领域。用于生物应用的DNA纳米管器件的小型化需要充分了解DNA与纳米管的相互作用机制。我们首次在此报告，单壁碳纳米管诱导的DNA去稳定化和构象转变是序列依赖性的。观察到单壁碳纳米管与聚[dGdC]：聚[dGdC]和聚[dAdT]：聚[dAdT]结合时的对比变化。对于GC同聚物，单壁碳纳米管使DNA解链温度降低了40摄氏度，但对AT-DNA没有影响。单壁碳纳米管可诱导GC-DNA发生B-A转变，但AT-DNA能抵抗这种转变。我们的圆二色性、竞争性结合测定和三链体去稳定化研究提供了直接证据，表明单壁碳纳米管在溶液中诱导DNA发生B-A转变，并且它们以GC偏好性结合到DNA大沟中。
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