两种依赖GacA的小RNA调节铜绿假单胞菌的群体感应反应。
Two GacA-dependent small RNAs modulate the quorum-sensing response in Pseudomonas aeruginosa.
作者信息
Kay Elisabeth, Humair Bérénice, Dénervaud Valérie, Riedel Kathrin, Spahr Stéphanie, Eberl Leo, Valverde Claudio, Haas Dieter
机构信息
Département de Microbiologie Fondamentale, Université de Lausanne, Building Biophore, CH-1015 Lausanne, Switzerland.
出版信息
J Bacteriol. 2006 Aug;188(16):6026-33. doi: 10.1128/JB.00409-06.
Abstract
In Pseudomonas aeruginosa, the GacS/GacA two-component system positively controls the quorum-sensing machinery and the expression of extracellular products via two small regulatory RNAs, RsmY and RsmZ. An rsmY rsmZ double mutant and a gacA mutant were similarly impaired in the synthesis of the quorum-sensing signal N-butanoyl-homoserine lactone, the disulfide bond-forming enzyme DsbA, and the exoproducts hydrogen cyanide, pyocyanin, elastase, chitinase (ChiC), and chitin-binding protein (CbpD). Both mutants showed increased swarming ability, azurin release, and early biofilm development.
摘要
在铜绿假单胞菌中，GacS/GacA双组分系统通过两个小调控RNA（RsmY和RsmZ）正向调控群体感应机制和细胞外产物的表达。rsmY rsmZ双突变体和gacA突变体在群体感应信号N-丁酰高丝氨酸内酯、二硫键形成酶DsbA以及细胞外产物氰化氢、绿脓菌素、弹性蛋白酶、几丁质酶（ChiC）和几丁质结合蛋白（CbpD）的合成方面同样受损。这两种突变体均表现出群游能力增强、天青蛋白释放增加以及生物膜早期形成。
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