通过长链分析基因表达技术（LongSAGE）检测到大量新的人类反义转录本。
A large quantity of novel human antisense transcripts detected by LongSAGE.
作者信息
Ge Xijin, Wu Qingfa, Jung Yong-Chul, Chen Jun, Wang San Ming
机构信息
Center for Functional Genomics, Division of Medical Genetics, Department of Medicine, ENH Research Institute, Northwestern University Feinberg School of Medicine 1001 University Place, Evanston, IL 60201 USA.
出版信息
Bioinformatics. 2006 Oct 15;22(20):2475-9. doi: 10.1093/bioinformatics/btl429. Epub 2006 Aug 7.
MOTIVATION
Taking advantage of the high sensitivity and specificity of LongSAGE tag for transcript detection and genome mapping, we analyzed the 632 813 unique human LongSAGE tags deposited in public databases to identify novel human antisense transcripts.
RESULTS
Our study identified 45 321 tags that match the antisense strand of 9804 known mRNA sequences, 6606 of which contain antisense ESTs and 3198 are mapped only by SAGE tags. Quantitative analysis showed that the detected antisense transcripts are present at levels lower than their counterpart sense transcripts. Experimental results confirmed the presence of antisense transcripts detected by the antisense tags. We also constructed an antisense tag database that can be used to identify the antisense SAGE tags originated from the antisense strand of known mRNA sequences included in the RefSeq database.
CONCLUSIONS
Our study highlights the benefits of exploring SAGE data for comprehensive identification of human antisense transcripts and demonstrates the prevalence of antisense transcripts in the human genome.
研究动机
利用LongSAGE标签在转录本检测和基因组定位方面的高灵敏度和特异性，我们分析了公共数据库中存放的632813个独特的人类LongSAGE标签，以识别新的人类反义转录本。
研究结果
我们的研究识别出45321个与9804个已知mRNA序列的反义链匹配的标签，其中6606个包含反义EST，3198个仅由SAGE标签定位。定量分析表明，检测到的反义转录本的水平低于其对应的正义转录本。实验结果证实了反义标签检测到的反义转录本的存在。我们还构建了一个反义标签数据库，可用于识别源自RefSeq数据库中已知mRNA序列反义链的反义SAGE标签。
研究结论
我们的研究突出了探索SAGE数据以全面识别人类反义转录本的益处，并证明了反义转录本在人类基因组中的普遍性。
Zotero 插件