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新种阿拉伊戈登氏菌和新种扩散戈登氏菌,从日本患者体内分离得到。

Gordonia araii sp. nov. and Gordonia effusa sp. nov., isolated from patients in Japan.

作者信息

Kageyama Akiko, Iida Soji, Yazawa Katsukiyo, Kudo Takuji, Suzuki Shin-Ichi, Koga Takeharu, Saito Hiromi, Inagawa Hiroko, Wada Akihito, Kroppenstedt Reiner M, Mikami Yuzuru

机构信息

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.

Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.

出版信息

Int J Syst Evol Microbiol. 2006 Aug;56(Pt 8):1817-1821. doi: 10.1099/ijs.0.64067-0.

Abstract

Two bacterial strains, IFM 10211(T) and IFM 10200(T), were isolated from the sputum of two Japanese patients, and were subjected to a polyphasic taxonomic study. The two strains were found to have morphological, physiological and chemotaxonomic properties that were consistent with their assignment to the genus Gordonia, except for a few chemotaxonomic characteristics. Almost complete 16S rRNA gene sequences of the two strains were determined; the data showed that they are related distantly to Gordonia amarae, Gordonia hirsuta, Gordonia hydrophobica and Gordonia sihwensis, showing 16S rRNA gene sequence similarities to the type strains of these species of 96.2-97.9 %. DNA-DNA relatedness data coupled with the combination of genotypic and phenotypic data indicated that the two strains are representatives of two novel, separate species. The names proposed to accommodate these two strains are Gordonia araii sp. nov. (type strain IFM 10211(T)=DSM 44811(T)=NBRC 100433(T)=JCM 12131(T)) and Gordonia effusa sp. nov. (type strain IFM 10200(T)=DSM 44810(T)=NBRC 100432(T)=JCM 12130(T)).

摘要

从两名日本患者的痰液中分离出两株细菌菌株,IFM 10211(T) 和IFM 10200(T),并对其进行了多相分类研究。发现这两株菌株具有形态、生理和化学分类学特性,除了一些化学分类学特征外,这些特性与其被归入戈登氏菌属相符。测定了这两株菌株几乎完整的16S rRNA基因序列;数据显示它们与苦戈登氏菌、多毛戈登氏菌、疏水戈登氏菌和丝滑戈登氏菌的亲缘关系较远,与这些菌种模式菌株的16S rRNA基因序列相似性为96.2 - 97.9%。DNA - DNA相关性数据以及基因型和表型数据的结合表明,这两株菌株是两个新的、独立的菌种的代表。为容纳这两株菌株而提议的名称分别是新种荒井戈登氏菌(模式菌株IFM 10211(T)=DSM 44811(T)=NBRC 100433(T)=JCM 12131(T))和新种疏松戈登氏菌(模式菌株IFM 10200(T)=DSM 44810(T)=NBRC 100432(T)=JCM 12130(T))。

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