Liu Ping, Wen Wei-Hua, Song Xiao-Xiao, Yuan Wei-Hong
School of Public Health, Tongji Medical College, Huazhong University for Science and Technology, Wuhan 430030, China.
Wei Sheng Yan Jiu. 2006 May;35(3):257-60.
To study dose-response relationship effects of mixed cypermethrin and methyl parathion on reproductive hormones, thyroid hormones, and immune functions in rats.
Eighty 2-month old Wistar rats (40 males and 40 females) were divided randomly by bodyweight into 4 groups. Four doses (0, 1/600 LD50, 1/135 LD50 and 1/30 LD50) were chosen for the combined exposure representing respective doses of cypermethrin 0, 0.4, 1.8 and 8.0 mg/kg body weight and of methylparathion 0, 0.0115, 0.0518 and 0.2300 mg/kg body weight. The control group received vehicle solvent only. All groups were force-fed every two days for 30 days with these dose combinations. Body weight gain and organ weights were determined. Serum levels of IgG and IgA, reproductive hormones (luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2), and testosterone), as well as the thyroid hormones (triiodothyronine (T3), tetraiodothyronine (T4), and thyroid stimulating hormone (TSH) were measured using radioimmunoassay (RIA). In addition, two immunological parameters (rate of neutrophil phagocytosis, rate of lymphocyte transformation) were being measured in blood samples.
The body weight gains were similar in all 4 groups. The weights of adrenal glands in exposed rats were heavier than those in control (P < 0.05). Serum FSH and E2 levels in exposed rats were higher than those in the control group (P < 0.01). Serum TSH levels were proportionally increasing with higher pesticide doses (r(s) = 0.329, P < 0.01). Lymphocyte transformation rates in all exposed animals were lower than that of the control group (P < 0.01). To the contrary, rates of neutrophil phagocytosis in all exposure groups were higher than those of the control group (P < 0.01). Furthermore, serum IgG levels of all exposed animals were lower than that of the control (P < 0.01) and serum IgA levels in exposed females were higher than that of the control (P < 0.01). Dose-response relationships for these changes were significant (rank correlation statistics P < 0.05 or < 0.01).
Our results showed that exposure to different mixtures of cypermethrin and methyl parathion disrupted the endocrine hormone levels, and immune functions in rats.
研究氯氰菊酯与甲基对硫磷混合物对大鼠生殖激素、甲状腺激素及免疫功能的剂量-反应关系效应。
将80只2月龄Wistar大鼠（40只雄性和40只雌性）按体重随机分为4组。选择4个剂量（0、1/600 LD50、1/135 LD50和1/30 LD50）进行联合暴露，分别代表氯氰菊酯剂量为0、0.4、1.8和8.0 mg/kg体重，甲基对硫磷剂量为0、0.0115、0.0518和0.2300 mg/kg体重。对照组仅给予赋形剂溶剂。所有组每两天强制灌胃一次，持续30天，给予这些剂量组合。测定体重增加量和器官重量。采用放射免疫分析法（RIA）测定血清IgG和IgA水平、生殖激素（促黄体生成素（LH）、促卵泡激素（FSH）、雌二醇（E2）和睾酮）以及甲状腺激素（三碘甲状腺原氨酸（T3）、四碘甲状腺原氨酸（T4）和促甲状腺激素（TSH））。此外，还测定了血液样本中的两个免疫参数（中性粒细胞吞噬率、淋巴细胞转化率）。
所有4组的体重增加量相似。暴露组大鼠的肾上腺重量比对照组重（P < 0.05）。暴露组大鼠血清FSH和E2水平高于对照组（P < 0.01）。血清TSH水平随农药剂量增加而呈比例升高（r(s)=0.329，P < 0.01）。所有暴露动物的淋巴细胞转化率均低于对照组（P < 0.01）。相反，所有暴露组的中性粒细胞吞噬率均高于对照组（P < 0.01）。此外，所有暴露动物的血清IgG水平低于对照组（P < 0.01），暴露雌性大鼠的血清IgA水平高于对照组（P < 0.01）。这些变化的剂量-反应关系具有显著性（秩相关统计P < 0.05或< 0.01）。
我们的数据表明，暴露于不同组合的氯氰菊酯和甲基对硫磷会扰乱大鼠的内分泌激素水平和免疫功能。
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