Kita Yoshihiro, Ohto Takayo, Uozumi Naonori, Shimizu Takao
Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
Biochim Biophys Acta. 2006 Nov;1761(11):1317-22. doi: 10.1016/j.bbalip.2006.08.001. Epub 2006 Aug 3.
Phospholipase A(2) (PLA(2)) (EC 3.1.1.4) catalyzes hydrolysis of the sn-2 ester bond of glycerophospholipids. The enzyme is essential for the production of two classes of lipid mediators, fatty acid metabolites and lysophospholipid-related lipids, as well as being involved in the remodeling of membrane phospholipids. Among many mammalian PLA(2)s, cytosolic PLA(2)alpha (cPLA(2)alpha) plays a critical role in various physiological and pathophysiological conditions through generating lipid mediators. Here, we summarize the in vivo significance of cPLA(2)alpha, revealed from the phenotypes of cPLA(2)alpha-null mice, and properties of newly discovered cPLA(2) family enzymes. We also briefly introduce a quantitative lipidomics strategy using liquid chromatography-mass spectrometry, a powerful tool for the comprehensive analysis of lipid mediators.
磷脂酶A(2)（PLA(2)）（EC 3.1.1.4）催化甘油磷脂sn-2酯键的水解。该酶对于两类脂质介质、脂肪酸代谢产物和溶血磷脂相关脂质的产生至关重要，并且参与膜磷脂的重塑。在众多哺乳动物PLA(2)中，胞质型磷脂酶A(2)α（cPLA(2)α）通过生成脂质介质在各种生理和病理生理条件下起关键作用。在此，我们总结了从cPLA(2)α基因敲除小鼠的表型揭示的cPLA(2)α的体内意义，以及新发现的cPLA(2)家族酶的特性。我们还简要介绍了一种使用液相色谱 - 质谱联用的定量脂质组学策略，这是一种用于脂质介质综合分析的强大工具。
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