Hedrén Marie, Ballagi Andras, Mörtsell Lars, Rajkai Gyogy, Stenmark Pål, Sturesson Christer, Nordlund Pär
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1227-31. doi: 10.1107/S090744490603441X. Epub 2006 Sep 19.
As part of the Structural Proteomics In Europe (SPINE) project, the automated multifermenter system GRETA has been developed with structural genomics as a major application. GRETA comprises 6-24 parallel fermentation chambers, each with individual control of fermentation parameters such as temperature, stirring, pH, dissolved oxygen concentration and feed profiles. Six human proteins were used to optimize the GRETA fermentation processes and to compare these processes with typical baffled-flask protocols used in structural genomics projects. The optimized GRETA processes allows several times more protein to be produced per litre of culture with limited manual intervention and constitutes a potentially useful alternative both for scale-up production in structural proteomic projects and for fermentation-process optimization.
作为欧洲结构蛋白质组学（SPINE）项目的一部分，已开发出以结构基因组学为主要应用的自动化多发酵罐系统GRETA。GRETA由6至24个平行发酵室组成，每个发酵室可单独控制发酵参数，如温度、搅拌、pH值、溶解氧浓度和进料曲线。使用了六种人类蛋白质来优化GRETA发酵过程，并将这些过程与结构基因组学项目中使用的典型摇瓶方案进行比较。优化后的GRETA工艺在人工干预有限的情况下，每升培养物可产生数倍于以往的蛋白质，对于结构蛋白质组学项目的扩大生产和发酵工艺优化而言，都是一种潜在的有用替代方案。
