Wang Chien-Yu, Chiao Ming-Tsang, Yen Po-Jen, Huang Wei-Chou, Hou Chia-Chung, Chien Shih-Chang, Yeh Kuo-Chen, Yang Wen-Ching, Shyur Lie-Fen, Yang Ning-Sun
Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China.
Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China.
Genomics. 2006 Dec;88(6):801-808. doi: 10.1016/j.ygeno.2006.08.011. Epub 2006 Sep 29.
Echinacea spp. are popularly used as an herbal medicine or food supplement for enhancing the immune system. This study shows that plant extracts from root [R] and stem plus leaf [S+L] tissues of E. purpurea exhibit opposite (enhancing vs inhibitory) modulatory effects on the expression of the CD83 marker in human dendritic cells (DCs), which are known as professional antigen-presenting cells. We developed a function-targeted DNA microarray system to characterize the effects of phytocompounds on human DCs. Down-regulation of mRNA expression of specific chemokines (e.g., CCL3 and CCL8) and their receptors (e.g., CCR1 and CCR9) was observed in [S+L]-treated DCs. Other chemokines and regulatory molecules (e.g., CCL4 and CCL2) involved in the c-Jun pathway were found to be up-regulated in [R]-treated DCs. This study, for the first time, demonstrates that E. purpurea extracts can modulate DC differentiation and expression of specific immune-related genes in DCs.
紫锥菊属植物被广泛用作增强免疫系统的草药或食品补充剂。本研究表明，紫锥菊根[R]以及茎加叶[S+L]组织的植物提取物对人树突状细胞（DCs，即专业抗原呈递细胞）中CD83标志物的表达呈现相反（增强与抑制）的调节作用。我们开发了一种功能靶向DNA微阵列系统来表征植物化合物对人DCs的影响。在[S+L]处理的DCs中观察到特定趋化因子（如CCL3和CCL8）及其受体（如CCR1和CCR9）的mRNA表达下调。发现参与c-Jun途径的其他趋化因子和调节分子（如CCL4和CCL2）在[R]处理的DCs中上调。本研究首次证明紫锥菊提取物可调节DC分化以及DCs中特定免疫相关基因的表达。
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