Gregor Jakub, Marsálek Blahoslav, Sípková Helena
Centre for Cyanobacteria and Their Toxins, Institute of Botany, Academy of Sciences of the Czech Republic, Kvĕtná 8, 603 65 Brno, Czech Republic.
Water Res. 2007 Jan;41(1):228-34. doi: 10.1016/j.watres.2006.08.011. Epub 2006 Oct 2.
In vivo fluorescence methods have been accepted as a quick, simple, and useful tool for quantification of phytoplankton organisms. In this paper, we present a case study in which fluorescence methods were employed for the selective detection of potentially toxic cyanobacteria in raw water at the drinking water treatment plant. The occurrence of cyanobacteria in the drinking water reservoir and in raw water was monitored by phycocyanin fluorescence measurements and by standard methods for phytoplankton quantification (cell counts, chlorophyll a). A special attention was paid to the most critical parts of the season -- spring recruitment of cyanobacteria from sediment to water column and autumn bloom collapse. All methods showed similar patterns within the season. Phycocyanin fluorescence was found to be a simple and sensitive indicator of cyanobacteria in water and can serve as a tool that can provide an early warning about the presence of potentially toxic cyanobacterial metabolites in water.
体内荧光法已被公认为是一种用于定量浮游植物生物的快速、简单且有用的工具。在本文中,我们展示了一个案例研究,其中荧光法被用于饮用水处理厂原水中潜在有毒蓝藻的选择性检测。通过藻蓝蛋白荧光测量以及浮游植物定量的标准方法(细胞计数、叶绿素a)监测饮用水水库和原水中蓝藻的出现情况。特别关注了该季节最关键的时期——春季蓝藻从沉积物向水柱的复苏以及秋季水华的崩溃。所有方法在该季节内都呈现出相似的模式。发现藻蓝蛋白荧光是水中蓝藻的一种简单且灵敏的指标,可作为一种能够对水中潜在有毒蓝藻代谢产物的存在发出早期预警的工具。