Kvist Thomas, Ahring Birgitte K, Westermann Peter
Bioprocess Science and Technology Group, Biocentrum-DTU Technical University of Denmark, Lyngby, Denmark.
FEMS Microbiol Ecol. 2007 Jan;59(1):71-80. doi: 10.1111/j.1574-6941.2006.00209.x. Epub 2006 Oct 2.
Whole-cell density gradient extractions from three solfataras (pH 2.5) ranging in temperature from 81 to 90 degrees C and one neutral hot spring (81 degrees C, pH 7) from the thermal active area of Hveragerethi (Iceland) were analysed for genetic diversity and local geographical variation of Archaea by analysis of amplified 16S rRNA genes. In addition to the three solfataras and the neutral hot spring, 10 soil samples in transects of the soil adjacent to the solfataras were analysed using terminal restriction fragment length polymorphism (t-RFLP). The sequence data from the clone libraries in combination with 14 t-RFLP profiles revealed a high abundance of clones clustering together with sequences from the nonthermophilic I.1b group of Crenarchaeota. The archaeal diversity in one solfatara was high; 26 different RFLP patterns were found using double digestion of the PCR products with restriction enzymes AluI and BsuRI. The sequenced clones from this solfatara belonged to Sulfolobales, Thermoproteales or were most closest related to sequences from uncultured Archaea. Sequences related to group I.1b were not found in the neutral hot spring or the hyperthermophilic solfatara (90 degrees C).
对来自冰岛赫伊拉盖蒂热活动区的3个温度在81至90摄氏度之间的酸性热泉（pH 2.5）和1个中性温泉（81摄氏度，pH 7）进行全细胞密度梯度提取，通过分析扩增的16S rRNA基因来研究古菌的遗传多样性和局部地理变异。除了这3个酸性热泉和中性温泉外，还使用末端限制性片段长度多态性（t-RFLP）分析了酸性热泉附近土壤样带中的10个土壤样本。来自克隆文库的序列数据与14个t-RFLP图谱相结合，揭示了大量与泉古菌门非嗜热I.1b组序列聚类在一起的克隆。其中一个酸性热泉中的古菌多样性很高；使用限制性内切酶AluI和BsuRI对PCR产物进行双酶切，发现了26种不同的RFLP模式。来自该酸性热泉的测序克隆属于硫化叶菌目、热变形菌目，或与未培养古菌的序列关系最为密切。在中性温泉或超嗜热酸性热泉（90摄氏度）中未发现与I.1b组相关的序列。
