一组M型共有包膜糖蛋白可诱导产生能中和B亚型和C亚型HIV-1原代病毒亚群的抗体。
A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses.
作者信息
Liao Hua-Xin, Sutherland Laura L, Xia Shi-Mao, Brock Mary E, Scearce Richard M, Vanleeuwen Stacie, Alam S Munir, McAdams Mildred, Weaver Eric A, Camacho Zenaido, Ma Ben-Jiang, Li Yingying, Decker Julie M, Nabel Gary J, Montefiori David C, Hahn Beatrice H, Korber Bette T, Gao Feng, Haynes Barton F
机构信息
Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
出版信息
Virology. 2006 Sep 30;353(2):268-82. doi: 10.1016/j.virol.2006.04.043.
Abstract
HIV-1 subtype C is the most common HIV-1 group M subtype in Africa and many parts of Asia. However, to date HIV-1 vaccine candidate immunogens have not induced potent and broadly neutralizing antibodies against subtype C primary isolates. We have used a centralized gene strategy to address HIV-1 diversity and generated a group M consensus envelope gene with shortened consensus variable loops (CON-S) for comparative studies with wild-type (WT) Env immunogens. Our results indicate that the consensus HIV-1 group M CON-S Env elicited cross-subtype neutralizing antibodies of similar or greater breadth and titer than the WT Envs tested, indicating the utility of a centralized gene strategy. Our study also shows the feasibility of iterative improvements in Env immunogenicity by rational design of centralized genes.
摘要
HIV-1 C亚型是非洲和亚洲许多地区最常见的HIV-1 M组亚型。然而，迄今为止，HIV-1候选疫苗免疫原尚未诱导出针对C亚型原始分离株的强效且广泛中和的抗体。我们采用了集中化基因策略来应对HIV-1的多样性，并生成了一个具有缩短的共有可变环（CON-S）的M组共有包膜基因，用于与野生型（WT）Env免疫原进行比较研究。我们的结果表明，共有HIV-1 M组CON-S Env诱导出的交叉亚型中和抗体的广度和效价与所测试的WT Env相似或更高，这表明集中化基因策略的实用性。我们的研究还表明，通过对集中化基因进行合理设计来迭代改善Env免疫原性是可行的。
相似文献
1
A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses.一组M型共有包膜糖蛋白可诱导产生能中和B亚型和C亚型HIV-1原代病毒亚群的抗体。
Virology. 2006 Sep 30;353(2):268-82. doi: 10.1016/j.virol.2006.04.043.
2
Ancestral and consensus envelope immunogens for HIV-1 subtype C.HIV-1 C亚型的祖传和共有包膜免疫原。
Virology. 2006 Sep 1;352(2):438-49. doi: 10.1016/j.virol.2006.05.011. Epub 2006 Jun 14.
3
Antigenicity and immunogenicity of HIV-1 consensus subtype B envelope glycoproteins.HIV-1 B亚型共有包膜糖蛋白的抗原性和免疫原性
Virology. 2007 Mar 30;360(1):218-34. doi: 10.1016/j.virol.2006.10.017. Epub 2006 Nov 13.
4
Evaluation of envelope vaccines derived from the South African subtype C human immunodeficiency virus type 1 TV1 strain.源自南非1型人类免疫缺陷病毒C亚型TV1株的包膜疫苗评估。
J Virol. 2005 Nov;79(21):13338-49. doi: 10.1128/JVI.79.21.13338-13349.2005.
5
Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies.来自急性和早期B亚型感染的1型人类免疫缺陷病毒env克隆，用于疫苗诱导中和抗体的标准化评估。
J Virol. 2005 Aug;79(16):10108-25. doi: 10.1128/JVI.79.16.10108-10125.2005.
6
Global panel of HIV-1 Env reference strains for standardized assessments of vaccine-elicited neutralizing antibodies.用于疫苗诱导中和抗体标准化评估的全球HIV-1 Env参考毒株专家组
J Virol. 2014 Mar;88(5):2489-507. doi: 10.1128/JVI.02853-13. Epub 2013 Dec 18.
7
Similar T-cell immune responses induced by group M consensus env immunogens with wild-type or minimum consensus variable regions.由具有野生型或最小共有可变区的M组共有env免疫原诱导的相似T细胞免疫反应。
AIDS Res Hum Retroviruses. 2010 May;26(5):577-84. doi: 10.1089/aid.2009.0258.
8
Comparison of HIV Type 1 ADA gp120 monomers versus gp140 trimers as immunogens for the induction of neutralizing antibodies.1型人类免疫缺陷病毒ADA株gp120单体与gp140三聚体作为诱导中和抗体免疫原的比较
AIDS Res Hum Retroviruses. 2005 Jan;21(1):58-67. doi: 10.1089/aid.2005.21.58.
9
Antigenicity and immunogenicity of transmitted/founder, consensus, and chronic envelope glycoproteins of human immunodeficiency virus type 1.人类免疫缺陷病毒 1 型传播/创始、共识和慢性包膜糖蛋白的抗原性和免疫原性。
J Virol. 2013 Apr;87(8):4185-201. doi: 10.1128/JVI.02297-12. Epub 2013 Jan 30.
10
The first hypervariable region of the gp120 Env glycoprotein defines the neutralizing susceptibility of heterologous human immunodeficiency virus type 1 isolates to neutralizing antibodies elicited by the SF162gp140 immunogen.gp120包膜糖蛋白的第一个高变区决定了异源1型人类免疫缺陷病毒分离株对由SF162gp140免疫原引发的中和抗体的中和敏感性。
J Virol. 2008 Jan;82(2):949-56. doi: 10.1128/JVI.02143-07. Epub 2007 Nov 14.
引用本文的文献
1
Computationally Selected Multivalent HIV-1 Subtype C Vaccine Protects Against Heterologous SHIV Challenge.通过计算筛选的多价HIV-1 C亚型疫苗可抵御异源SHIV攻击。
Vaccines (Basel). 2025 Feb 24;13(3):231. doi: 10.3390/vaccines13030231.
2
A Pentavalent HIV-1 Subtype C Vaccine Containing Computationally Selected gp120 Strains Improves the Breadth of V1V2 Region Responses.一种包含经计算筛选的gp120毒株的五价HIV-1 C亚型疫苗可提高V1V2区域反应的广度。
Vaccines (Basel). 2025 Jan 28;13(2):133. doi: 10.3390/vaccines13020133.
3
Mechanisms of sterilizing immunity provided by an HIV-1 neutralizing antibody against mucosal infection.一种HIV-1中和抗体提供的针对黏膜感染的杀菌免疫机制。
PLoS Pathog. 2024 Dec 26;20(12):e1012777. doi: 10.1371/journal.ppat.1012777. eCollection 2024 Dec.
4
Enhancement of Neutralization Responses through Sequential Immunization of Stable Env Trimers Based on Consensus Sequences from Select Time Points by Mimicking Natural Infection.通过模拟自然感染，基于选定时间点的共识序列对稳定的 Env 三聚体进行连续免疫接种，增强中和反应。
Int J Mol Sci. 2023 Aug 10;24(16):12642. doi: 10.3390/ijms241612642.
5
Adaptation of a transmitted/founder simian-human immunodeficiency virus for enhanced replication in rhesus macaques.传播/原始猴免疫缺陷病毒适应增强在恒河猴中的复制。
PLoS Pathog. 2023 Jul 3;19(7):e1011059. doi: 10.1371/journal.ppat.1011059. eCollection 2023 Jul.
6
Fine-mapping the immunodominant antibody epitopes on consensus sequence-based HIV-1 envelope trimer vaccine candidates.精细定位基于共有序列的HIV-1包膜三聚体疫苗候选物上的免疫显性抗体表位。
NPJ Vaccines. 2022 Nov 25;7(1):152. doi: 10.1038/s41541-022-00576-9.
7
Adenoviral-Vectored Centralized Consensus Hemagglutinin Vaccine Provides Broad Protection against H2 Influenza a Virus.腺病毒载体集中式共识血凝素疫苗对H2甲型流感病毒提供广泛保护。
Vaccines (Basel). 2022 Jun 10;10(6):926. doi: 10.3390/vaccines10060926.
8
Mouse and human antibodies bind HLA-E-leader peptide complexes and enhance NK cell cytotoxicity.鼠源和人源抗体结合 HLA-E-肽复合物并增强 NK 细胞的细胞毒性。
Commun Biol. 2022 Mar 28;5(1):271. doi: 10.1038/s42003-022-03183-5.
9
Rapid selection of HIV envelopes that bind to neutralizing antibody B cell lineage members with functional improbable mutations.快速选择与具有功能上不可能突变的中和抗体 B 细胞谱系成员结合的 HIV 包膜。
Cell Rep. 2021 Aug 17;36(7):109561. doi: 10.1016/j.celrep.2021.109561.
10
Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates.接种疫苗的非人类灵长类动物中 HIV-1 中和抗体的结构和遗传趋同。
PLoS Pathog. 2021 Jun 4;17(6):e1009624. doi: 10.1371/journal.ppat.1009624. eCollection 2021 Jun.
本文引用的文献
1
Evaluating neutralizing antibodies against HIV, SIV, and SHIV in luciferase reporter gene assays.在荧光素酶报告基因检测中评估针对HIV、SIV和SHIV的中和抗体。
Curr Protoc Immunol. 2005 Jan;Chapter 12:12.11.1-12.11.17. doi: 10.1002/0471142735.im1211s64.
2
Isolation and quantitation of HIV in peripheral blood.外周血中HIV的分离与定量
Curr Protoc Immunol. 2001 May;Chapter 12:12.2.1-12.2.11. doi: 10.1002/0471142735.im1202s05.
3
Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates.分析HIV-1 B亚型第三个可变区肽基序以诱导针对HIV-1原代分离株的中和抗体。
Virology. 2006 Feb 5;345(1):44-55. doi: 10.1016/j.virol.2005.08.042. Epub 2005 Oct 20.
4
Evaluation of envelope vaccines derived from the South African subtype C human immunodeficiency virus type 1 TV1 strain.源自南非1型人类免疫缺陷病毒C亚型TV1株的包膜疫苗评估。
J Virol. 2005 Nov;79(21):13338-49. doi: 10.1128/JVI.79.21.13338-13349.2005.
5
Polyvalent DNA prime and envelope protein boost HIV-1 vaccine elicits humoral and cellular responses and controls plasma viremia in rhesus macaques following rectal challenge with an R5 SHIV isolate.多价DNA初免和包膜蛋白加强的HIV-1疫苗在恒河猴经直肠接种R5 SHIV毒株攻击后可引发体液和细胞免疫反应并控制血浆病毒血症。
J Med Primatol. 2005 Oct;34(5-6):226-36. doi: 10.1111/j.1600-0684.2005.00120.x.
6
Human immunodeficiency virus type 1 subtype B ancestral envelope protein is functional and elicits neutralizing antibodies in rabbits similar to those elicited by a circulating subtype B envelope.人类免疫缺陷病毒1型B亚型祖传包膜蛋白具有功能，并在兔子体内引发中和抗体，类似于由循环B亚型包膜引发的中和抗体。
J Virol. 2005 Sep;79(17):11214-24. doi: 10.1128/JVI.79.17.11214-11224.2005.
7
Formaldehyde-treated, heat-inactivated virions with increased human immunodeficiency virus type 1 env can be used to induce high-titer neutralizing antibody responses.经甲醛处理、热灭活且具有增强的1型人类免疫缺陷病毒包膜的病毒粒子可用于诱导高滴度中和抗体反应。
J Virol. 2005 Aug;79(16):10210-7. doi: 10.1128/JVI.79.16.10210-10217.2005.
8
Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies.来自急性和早期B亚型感染的1型人类免疫缺陷病毒env克隆，用于疫苗诱导中和抗体的标准化评估。
J Virol. 2005 Aug;79(16):10108-25. doi: 10.1128/JVI.79.16.10108-10125.2005.
9
Recommendations for the design and use of standard virus panels to assess neutralizing antibody responses elicited by candidate human immunodeficiency virus type 1 vaccines.关于设计和使用标准病毒组以评估候选1型人类免疫缺陷病毒疫苗引发的中和抗体反应的建议。
J Virol. 2005 Aug;79(16):10103-7. doi: 10.1128/JVI.79.16.10103-10107.2005.
10
Application of the polyvalent approach to HIV-1 vaccine development.多价方法在HIV-1疫苗研发中的应用。
Curr Drug Targets Infect Disord. 2005 Jun;5(2):143-56. doi: 10.2174/1568005054201517.
Zotero 插件