用于快速检测牛病毒性腹泻病毒抗原的免疫层析检测试剂盒的研制
Development of an immunochromatographic test kit for rapid detection of bovine viral diarrhea virus antigen.
作者信息
Kameyama K, Sakoda Y, Tamai K, Igarashi H, Tajima M, Mochizuki T, Namba Y, Kida H
机构信息
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Sapporo 060-0818, Japan.
出版信息
J Virol Methods. 2006 Dec;138(1-2):140-6. doi: 10.1016/j.jviromet.2006.08.005. Epub 2006 Oct 12.
An immunochromatographic test was developed for rapid diagnosis of bovine viral diarrhea virus (BVDV) infections using monoclonal antibodies against the nonstructural protein, NS3, of the virus. The kit detected specifically the NS3 of various BVDV strains. Using the kit, leukocyte extracts of cattle infected persistently with BVDV were found positive while those of healthy cattle were negative. The sensitivity and specificity of this kit in compared with virus isolation were 100% and 97.2%, respectively. Furthermore, the test also gave positive results for calves infected acutely with BVDV in experimental infection. The BVDV antigen was detected in 1 ml of blood using a relatively simple procedure. This test kit should be useful for rapid diagnosis of BVD.
利用针对牛病毒性腹泻病毒（BVDV）非结构蛋白NS3的单克隆抗体，开发了一种免疫层析试验用于快速诊断BVDV感染。该试剂盒能特异性检测各种BVDV毒株的NS3。使用该试剂盒，发现持续感染BVDV的牛的白细胞提取物呈阳性，而健康牛的白细胞提取物呈阴性。与病毒分离相比，该试剂盒的敏感性和特异性分别为100%和97.2%。此外，在实验感染中，该试验对急性感染BVDV的犊牛也给出了阳性结果。采用相对简单的程序即可在1毫升血液中检测到BVDV抗原。该检测试剂盒应有助于BVD的快速诊断。
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