[水红花子质量研究]

[Study on quality for Herba Polygoni Orientalis].

作者信息

Wang Ai-min, Wang Yong-lin, Hu Li-na, Li Yong-jun

机构信息

School of Pharmacy, Guiyang Medical Colledge, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2006 Jul;31(13):1047-9.

PMID:17048596

Abstract

OBJECTIVE

To establish the qualitative and quantitative detective methods of Herba Polygoni Orientalis.

METHOD

Isorientin, orientin, protocatechuic acid and gallic acid in Herba Polygoni Orientalis were identified by TLC. The contents of isorientin and orientin in Herba Polygoni Orientalis were determined by HPLC.

RESULT

Isorientin, orientin, protocatechuic acid and gallic acid could be identified by TLC. Isorientin and orientin were well separated with Diamonsil C18 column and acetonitrile-0.1% phosphoric acid (18:82) as mobile phase. The linear range of isorientin was 0.075-0.90 microg. The average recovery of isorientin was 98.8% and RSD was 2.1%. The linear range of orientin was 0.041-0.49 microg. The average recovery of orientin was 98.8% and RSD was 2.1%.

CONCLUSION

The methods can be used for qualitative identification and quantitation determination of Herba Polygoni Orientalis.

摘要

目的

建立水红花子的定性及定量检测方法。

方法

采用薄层色谱法（TLC）对水红花子中的异荭草苷、荭草苷、原儿茶酸和没食子酸进行鉴别；采用高效液相色谱法（HPLC）测定水红花子中异荭草苷和荭草苷的含量。

结果

薄层色谱法可鉴别出水红花子中的异荭草苷、荭草苷、原儿茶酸和没食子酸。采用Diamonsil C18柱，以乙腈-0.1%磷酸（18:82）为流动相，异荭草苷和荭草苷得到良好分离。异荭草苷的线性范围为0.075 - 0.90μg，平均回收率为98.8%，相对标准偏差（RSD）为2.1%；荭草苷的线性范围为0.041 - 0.49μg，平均回收率为98.8%，RSD为2.1%。