虎杖抗癌活性物质的研究
[Studies on active substance of anticancer effect in Polygonum cuspidatum].
作者信息
Feng Lei, Zhang Lian-fen, Yan Ting, Jin Jian, Tao Wen-yi
机构信息
The Biopharmaceutical Laboratory, School of Biotechnology, Yangtze University, Wuxi, China.
出版信息
Zhong Yao Cai. 2006 Jul;29(7):689-91.
OBJECTIVE
To supply the scientific basis of research and development of the medicinal value of Polygonum cuspidatum.
METHODS
One composition was isolated from the roots of Polygonum cuspidatum by cytotoxicity based fractionation and identified by HPLC-MS, UV scanning and IR. The inhibition and morphology of L-02, Hep G2, SHZ-888, MCF-7, MCF-7/ADM cells growth caused by this composition was determined by MTT assay and HE dyeing.
RESULTS
This composition was identified as trans-and cis-resveratrol. It could specifically inhibit proliferation of many cancer cells but not human normal liver cell. We investigated the cytotoxicity of resveratrol to adriamycin-resistant MCF-7 cell in virtro.
CONCLUSION
Resveratrol is a new anticancer composition which is less toxicity and higher efficiency in Polygonum cuspidatum.
目的
为虎杖药用价值的研发提供科学依据。
方法
采用基于细胞毒性的分级分离法从虎杖根中分离出一种成分，并通过高效液相色谱-质谱联用（HPLC-MS）、紫外扫描和红外光谱进行鉴定。采用MTT法和苏木精-伊红（HE）染色法测定该成分对L-02、Hep G2、SHZ-888、MCF-7、MCF-7/ADM细胞生长的抑制作用及形态学变化。
结果
该成分鉴定为反式白藜芦醇和顺式白藜芦醇。它能特异性抑制多种癌细胞的增殖，但对人正常肝细胞无抑制作用。我们在体外研究了白藜芦醇对阿霉素耐药MCF-7细胞的细胞毒性。
结论
白藜芦醇是虎杖中一种新的抗癌成分，具有低毒高效的特点。
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