Lu Y Q, Liang X W, Zhang M, Wang W L, Kitiyanant Y, Lu S S, Meng B, Lu K H
Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, China.
Anim Reprod Sci. 2007 Jul;100(1-2):192-6. doi: 10.1016/j.anireprosci.2006.09.019. Epub 2006 Sep 15.
Flow-cytometric sorting of mammalian sperm for production of offspring with the desired sex is one of the most important new biotechnologies available for livestock industry. The objectives of this study were: (i) to sort the sperm into X- and Y-enriched populations and (ii) use the sorted sperm for in vitro fertilization (IVF) to produce sex-preselected embryo and offspring. The results revealed that the accuracy of sorted X- and Y-sperm was 94% and 89%, respectively. There was a decrease in blastocyst development rate in IVF with sorted sperm comparing to unsorted sperm, but the percentage of blastocysts on D6-D8 was not statistically different. Transplantation of the presumed X-embryos derived from IVF into a recipient resulted in the birth of female twins. These results indicated the feasibility of sperm sorting by flow cytometry and in vitro production of sex-preselected embryos and offspring in buffalo.
通过流式细胞术对哺乳动物精子进行分选以生产具有所需性别的后代，是畜牧业可用的最重要的新生物技术之一。本研究的目的是：（i）将精子分选为富含X和Y的群体，以及（ii）使用分选后的精子进行体外受精（IVF）以生产性别预选的胚胎和后代。结果显示，分选后的X精子和Y精子的准确率分别为94%和89%。与未分选的精子相比，分选后的精子进行体外受精时囊胚发育率有所下降，但在第6至8天的囊胚百分比在统计学上没有差异。将体外受精获得的推测为X胚胎移植到受体中，产下了雌性双胞胎。这些结果表明，在水牛中通过流式细胞术分选精子以及体外生产性别预选的胚胎和后代是可行的。
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