Xie De-Qiong, Gan Hua, Du Xiao-Gang, Li Zheng-Rong, Wu Jiang
Department of Nephrology, First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Nov;22(6):763-6.
To investigate the characterization of Th1/Th2 profile in patients with end-stage renal disease (ESRD) and the correlation with the apoptosis of the peripheral blood T cells; and to study the influence of different dialysis membranes on the apoptosis of T lymphocyte.
T cells from 10 non-dialyzed (ND) patients, 45 maintenance hemodialysis patients with cellulose acetate (CA) membrane, low-flux (PS-LF) and high-flux polusulfone (PS-HF) membrane, and 8 healthy volunteers (HC) were separated and stimulated with PHA for 24 hours in vitro. Then the apoptosis of T cells and supernatants levels of IFN-gamma and IL-4 were detected by Flow cytometry (FCM) and ELISA.
In ESRD patients, the expression of Annexin V in T lymphocyte was higher than that of group HC (P<0.05), group CA was higher than group PS-HF and PS-LF (P<0.05). The level of IFN-gamma of ESRD patients was decreased significant compared with that in group HC (P<0.05), and there was negative correlation. between the Annexin V and IFN-gamma. IL-4 was increased in ESRD patients (P<0.05) and it was positive correlated with Annexin V.
ESRD patients showed suppressed secretion of IFN-gamma, increased secretion of IL-4 and apoptosis of T lymphocytes.
探讨终末期肾病（ESRD）患者Th1/Th2细胞谱特征及其与外周血T细胞凋亡的相关性；研究不同透析膜对T淋巴细胞凋亡的影响。
分离10例未透析（ND）患者、45例使用醋酸纤维素（CA）膜、低通量（PS-LF）和高通量聚砜（PS-HF）膜的维持性血液透析患者以及8名健康志愿者（HC）的T细胞，体外经PHA刺激24小时。然后采用流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测T细胞凋亡以及IFN-γ和IL-4的上清液水平。
ESRD患者T淋巴细胞中膜联蛋白V的表达高于HC组（P<0.05），CA组高于PS-HF组和PS-LF组（P<0.05）。ESRD患者的IFN-γ水平与HC组相比显著降低（P<0.05），且膜联蛋白V与IFN-γ之间呈负相关。ESRD患者IL-4升高（P<0.05），且与膜联蛋白V呈正相关。
ESRD患者表现出IFN-γ分泌受抑制、IL-4分泌增加以及T淋巴细胞凋亡。
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